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Journal of Bacteriology, July 2000, p. 4044-4050, Vol. 182, No. 14
Department of Microbiology, University of
Washington, Seattle, Washington 98195,1 and
Izumi Campus, Meiji University, 1-9-1 Eifuku, Suginami,
Tokyo 168-0064, Japan2
Received 11 February 2000/Accepted 3 May 2000
We investigated the posttranscriptional regulation of
flgE, a class 2 gene that encodes the hook subunit protein
of the flagella. RNase protection assays demonstrated that the
flgE gene was transcribed at comparable levels in numerous
strains defective in known steps of flagellar assembly. However,
Western analyses of these strains demonstrated substantial differences
in FlgE protein levels. Although wild-type FlgE levels were observed in
strains with deletions of genes encoding components of the switch
complex and the flagellum-specific secretion apparatus, no protein was
detected in a strain with deletions of the rod, ring, and
hook-associated proteins. To determine whether FlgE levels were
affected by the stage of hook-basal-body assembly, Western analysis
was performed on strains with mutations at individual loci encompassed
by the deletion. FlgE protein was undetectable in rod mutants,
intermediate in ring mutants, and wild type in hook-associated protein
mutants. The lack of negative regulation in switch complex and
flagellum-specific secretion apparatus deletion mutants blocked for
flagellar construction prior to rod assembly suggests that these
structures play a role in the negative regulation of FlgE. Quantitative
Western analyses of numerous flagellar mutants indicate that FlgE
levels reflect the stage at which flagellar assembly is blocked. These
data provide evidence for negative posttranscriptional regulation of
FlgE in response to the stage of flagellar assembly.
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
The Flagellar Hook Protein, FlgE, of Salmonella
enterica Serovar Typhimurium Is Posttranscriptionally Regulated in
Response to the Stage of Flagellar Assembly
*
Corresponding author. Mailing address: Department of
Microbiology, University of Washington, Seattle, WA 98195. Phone: (206) 543-0129. Fax: (206) 543-8297. E-mail:
hughes{at}u.washington.edu.
Journal of Bacteriology, July 2000, p. 4044-4050, Vol. 182, No. 14
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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