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Journal of Bacteriology, July 2000, p. 4113-4116, Vol. 182, No. 14
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Genome Structure of the Genus Azospirillum

Claudia C. G. Martin-Didonet, Leda S. Chubatsu, Emanuel M. Souza, Margareth Kleina, Fabiane G. M. Rego, Liu U. Rigo, M. Geoffrey Yates, and Fabio O. Pedrosa*

Departamento de Bioquímica, Universidade Federal do Paraná, CEP-81531-990, Curitiba-PR, Brazil

Received 28 December 1999/Accepted 24 April 2000

Azospirillum species are plant-associated diazotrophs of the alpha subclass of Proteobacteria. The genomes of five of the six Azospirillum species were analyzed by pulsed-field gel electrophoresis. All strains possessed several megareplicons, some probably linear, and 16S ribosomal DNA hybridization indicated multiple chromosomes in genomes ranging in size from 4.8 to 9.7 Mbp. The nifHDK operon was identified in the largest replicon.

* Corresponding author. Mailing address: Departamento de Bioquímica, Universidade Federal do Paraná, Caixa Postal 19046, CEP-81531-990, Curitiba-PR, Brazil. Phone: 55 41 366 4398. Fax: 55 41 266 2042. E-mail: fpedrosa{at}bio.ufpr.br.

Journal of Bacteriology, July 2000, p. 4113-4116, Vol. 182, No. 14
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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