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Journal of Bacteriology, August 2000, p. 4173-4179, Vol. 182, No. 15
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Regulation of Acetyl Coenzyme A Synthetase in Escherichia coli

Suman Kumari,1,dagger Christine M. Beatty,1 Douglas F. Browning,2 Stephen J. W. Busby,2 Erica J. Simel,1 Galadriel Hovel-Miner,1 and Alan J. Wolfe1,*

Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois 60153,1 and School of Biosciences, The University of Birmingham, Birmingham B15 2TT, United Kingdom2

Received 7 January 2000/Accepted 8 May 2000

Cells of Escherichia coli growing on sugars that result in catabolite repression or amino acids that feed into glycolysis undergo a metabolic switch associated with the production and utilization of acetate. As they divide exponentially, these cells excrete acetate via the phosphotransacetylase-acetate kinase pathway. As they begin the transition to stationary phase, they instead resorb acetate, activate it to acetyl coenzyme A (acetyl-CoA) by means of the enzyme acetyl-CoA synthetase (Acs) and utilize it to generate energy and biosynthetic components via the tricarboxylic acid cycle and the glyoxylate shunt, respectively. Here, we present evidence that this switch occurs primarily through the induction of acs and that the timing and magnitude of this induction depend, in part, on the direct action of the carbon regulator cyclic AMP receptor protein (CRP) and the oxygen regulator FNR. It also depends, probably indirectly, upon the glyoxylate shunt repressor IclR, its activator FadR, and many enzymes involved in acetate metabolism. On the basis of these results, we propose that cells induce acs, and thus their ability to assimilate acetate, in response to rising cyclic AMP levels, falling oxygen partial pressure, and the flux of carbon through acetate-associated pathways.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Loyola University Chicago, Stritch School of Medicine, 2160 S. First Ave., Maguire Building 105, Rm. 3822, Maywood, IL 60153. Phone: (708) 216-5814. Fax: (708) 216-9574. E-mail: awolfe{at}luc.edu.

dagger Present address: Department of Oral Medicine and Diagnostic Sciences, Harvard School of Dental Medicine, Boston, MA 02115.

Journal of Bacteriology, August 2000, p. 4173-4179, Vol. 182, No. 15
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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