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Journal of Bacteriology, August 2000, p. 4343-4347, Vol. 182, No. 15
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Construction and Use of Derivatives of Transposon Tn4001 That Function in Mycoplasma pulmonis and Mycoplasma arthritidis

Kevin Dybvig,* C. Todd French, and LeRoy L. Voelkerdagger

Department of Comparative Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294

Received 16 February 2000/Accepted 18 April 2000

Previous attempts to introduce transposon Tn4001 into Mycoplasma pulmonis and Mycoplasma arthritidis have not been successful, possibly due to functional failure of the transposon's gentamicin resistance determinant. Tn4001C and Tn4001T were constructed, respectively, by insertion of a chloramphenicol acetyltransferase gene and the tetM tetracycline resistance determinant into Tn4001. Both Tn4001C and Tn4001T transposed in M. pulmonis, and Tn4001T transposed in M. arthritidis. The incorporation of a Tn4001T derivative that contained lacZ into either Mycoplasma species resulted in transformants with readily detectable LacZ activity. Tn4001T may be of general utility for use as a mycoplasma cloning vehicle because tetM functions in all species of Mycoplasma examined thus far.

* Corresponding author. Mailing address: Department of Comparative Medicine, University of Alabama at Birmingham, Volker Hall, Room 418A, Birmingham, AL 35294-0019. Phone: (205) 934-9327. Fax: (205) 975-4418. E-mail: dybvig{at}uab.edu.

dagger Present address: SmithKline Beecham Pharmaceuticals, Collegeville, PA 19426.

Journal of Bacteriology, August 2000, p. 4343-4347, Vol. 182, No. 15
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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