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Journal of Bacteriology, August 2000, p. 4348-4351, Vol. 182, No. 15
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Genetic Analysis of Sliding Motility in Mycobacterium smegmatis

Judith Recht, Asunción Martínez, Sandra Torello, and Roberto Kolter*

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115

Received 4 November 1999/Accepted 8 May 2000

A screen for nonsliding mutants of Mycobacterium smegmatis yielded 20 mutants with transposon insertions in the mps gene, which is involved in glycopeptidolipid biosynthesis. One mutant had an insertion in a gene predicted to encode a membrane transport protein. All mutants lacked glycopeptidolipids and were unable to form biofilms on polyvinyl chloride.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115. Phone: (617) 432-1776. Fax: (617) 738-7664. E-mail: kolter{at}mbcrr.harvard.edu.

Journal of Bacteriology, August 2000, p. 4348-4351, Vol. 182, No. 15
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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