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Journal of Bacteriology, August 2000, p. 4352-4355, Vol. 182, No. 15
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of a Second Region of the Spo0A Response Regulator of Bacillus subtilis Required for Transcription Activation

Dean A. Rowe-Magnus,1,dagger Martin J. Richer,1 and George B. Spiegelman1,2,*

Departments of Microbiology and Immunology1 and Medical Genetics,2 University of British Columbia, Vancouver, Canada V6T 1Z3

Received 13 September 1999/Accepted 11 May 2000

Deletion of the 10 C-terminal amino acids of the Bacillus subtilis response regulator Spo0A or valine substitution at D258 and L260 resulted in a sporulation-negative phenotype and loss of in vivo activation of the spoIIG and spoIIA operon promoters. Repression of the abrB promoter was not affected by the mutations. In combination with the previously characterized mutation (A257V), the results identify amino acids at positions 257, 258, and 260 as being required for transcription activation by Spo0A.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada V6T 1Z3. Phone: (604) 822-2036. Fax: (604) 822-6041. E-mail: spie{at}interchange.ubc.ca.

dagger Present address: Institut Pasteur, UPMTG-Department de Biotechnologie, 757724 Paris, France.

Journal of Bacteriology, August 2000, p. 4352-4355, Vol. 182, No. 15
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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