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Journal of Bacteriology, August 2000, p. 4401-4405, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Functional Characterization of the HasAPF Hemophore and Its Truncated and Chimeric Variants: Determination of a Region Involved in Binding to the Hemophore Receptor

Sylvie Létoffé,1 Kenji Omori,2 and Cécile Wandersman1,*

Unité des Membranes Bactériennes, Institut Pasteur (CNRS URA2172), 75724 Paris Cedex 15, France,1 and Discovery Research Laboratory Tanabe, Seiyaku Co. Ltd., Osaka 532-8505, and Toda, Saitama 335-8505, Japan2

Received 2 March 2000/Accepted 29 May 2000

Hemophores are secreted by several gram-negative bacteria (Serratia marcescens, Pseudomonas aeruginosa, Pseudomonas fluorescens, and Yersinia pestis) and form a family of homologous proteins. Unlike the S. marcescens hemophore (HasASM), the P. fluorescens hemophore HasAPF has an additional region of 12 residues located immediately upstream from the C-terminal secretion signal. We show that HasAPF undergoes a C-terminal cleavage which removes the last 21 residues when secreted from P. fluorescens and that only the processed form is able to deliver heme to the S. marcescens outer membrane hemophore-specific receptor, HasRSM. Functional analysis of variants including those with an internal deletion of the extra C-terminal domain show that the secretion signal does not inhibit the biological activity, whereas the 12-amino-acid region located upstream does. This extra domain may inhibit the interaction of the hemophore with HasRSM. To localize the hemophore regions involved in binding to HasR, chimeric HasAPF-HasASM proteins were tested for biological activity. We show that residues 153 to 180 of HasAPF are necessary for its interaction with the receptor.

* Corresponding author. Mailing address: Unité des Membranes Bactériennes, Institut Pasteur (CNRS URA2172), 25 rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: 33140613275. Fax: 33145688790. E-mail: cwander{at}pasteur.fr.

Journal of Bacteriology, August 2000, p. 4401-4405, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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