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Journal of Bacteriology, August 2000, p. 4414-4424, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of LrpC DNA-Binding Properties and Regulation of Bacillus subtilis lrpC Gene Expression

Christophe Beloin,1,dagger Rachel Exley,1 Anne-Laure Mahé,1 Mohamed Zouine,1 Stephanie Cubasch,2 and Françoise Le Hégarat1,*

Institut de Génétique et Microbiologie, Université Paris XI, 91405 Orsay Cedex, France,1 and Institut für Genetik, Ludwig Maximilians Universität, 80638 Munich, Germany2

Received 7 February 2000/Accepted 26 May 2000

The lrpC gene was identified during the Bacillus subtilis genome sequencing project. Previous experiments suggested that LrpC has a role in sporulation and in the regulation of amino acid metabolism and that it shares features with Escherichia coli Lrp, a transcription regulator (C. Beloin, S. Ayora, R. Exley, L. Hirschbein, N. Ogasawara, Y. Kasahara, J. C. Alonso, and F. Le Hégarat, Mol. Gen. Genet. 256:63-71, 1997). To characterize the interactions of LrpC with DNA, the protein was overproduced and purified. We show that LrpC binds to multiple sites in the upstream region of its own gene with a stronger affinity for a region encompassing P1, one of the putative promoters identified (P1 and P2). By analyzing lrpC-lacZ transcriptional fusions, we demonstrated that P1 is the major in vivo promoter and that, unlike many members of the lrp/asnC family, lrpC is not negatively autoregulated but rather slightly positively autoregulated. Production of LrpC in vivo is low in both rich and minimal media (50 to 300 LrpC molecules per cell). In rich medium, the cellular LrpC content is six- to sevenfold lower during the exponentional phase than during the stationary growth phase. Possible determinants and the biological significance of the regulation of lrpC expression are discussed.

* Corresponding author. Mailing address: Institut de Génétique et Mikrobiologie, Université Paris XI, Bât. 409, 91405 Orsay Cedex, France. Phone: 33 1 69 15 63 62. Fax: 33 1 69 15 78 08. E-mail: Francoise.Le-Hegarat{at}igmors.u-psud.fr.

dagger Present address: Moyne Institute of Preventive Medicine, Department of Microbiology, Trinity College, Dublin 2, Republic of Ireland.

Journal of Bacteriology, August 2000, p. 4414-4424, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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