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Journal of Bacteriology, August 2000, p. 4458-4465, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Global Gene Expression Profiles of Bacillus subtilis Grown under Anaerobic Conditions

Rick W. Ye,* Wang Tao, Laura Bedzyk, Thomas Young, Mario Chen, and Liao Li

Experimental Station E328/148B, DuPont Central Research and Development, Wilmington, Delaware 19880

Received 19 January 2000/Accepted 1 June 2000

Bacillus subtilis can grow under anaerobic conditions, either with nitrate or nitrite as the electron acceptor or by fermentation. A DNA microarray containing 4,020 genes from this organism was constructed to explore anaerobic gene expression patterns on a genomic scale. When mRNA levels of aerobic and anaerobic cultures during exponential growth were compared, several hundred genes were observed to be induced or repressed under anaerobic conditions. These genes are involved in a variety of cell functions, including carbon metabolism, electron transport, iron uptake, antibiotic production, and stress response. Among the highly induced genes are not only those responsible for nitrate respiration and fermentation but also those of unknown function. Certain groups of genes were specifically regulated during anaerobic growth on nitrite, while others were primarily affected during fermentative growth, indicating a complex regulatory circuitry of anaerobic metabolism.

* Corresponding author. Mailing address: Experimental Station E328/148B, DuPont Central Research and Development, Route 141 and Henry Clay Rd., Wilmington, DE 19880. Phone: (302) 695-1750. Fax: (302) 695-1829. E-mail: rick.ye{at}usa.dupont.com.

Journal of Bacteriology, August 2000, p. 4458-4465, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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