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Journal of Bacteriology, August 2000, p. 4628-4631, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Carboxy-Terminal 16-Amino-Acid Region of sigma 38 of Escherichia coli Is Important for Transcription under High-Salt Conditions and Sigma Activities In Vivo

Mio Ohnuma,1 Nobuyuki Fujita,2 Akira Ishihama,2 Kan Tanaka,1 and Hideo Takahashi1,*

Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-0032,1 and Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411-8540,2 Japan

Received 11 February 2000/Accepted 15 May 2000

sigma 38 (or sigma S, the rpoS gene product) is a sigma subunit of RNA polymerase in Escherichia coli and directs transcription from a number of stationary-phase promoters as well as osmotically inducible promoters. In this study, we analyzed the function of the carboxy-terminal 16-amino-acid region of sigma 38 (residues 315 to 330), which is well conserved among the rpoS gene products of enteric bacterial species. Truncation of this region was shown to result in the loss of sigma activity in vivo using promoter-lacZ fusion constructs, but the mutant sigma 38 retained the binding activity in vivo to the core enzyme. The in vitro transcription analysis revealed that the transcription activity of sigma 38 holoenzyme under high potassium glutamate concentrations was significantly decreased by the truncation of the carboxy-terminal tail element.

* Corresponding author. Mailing address: Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-0032, Japan. Phone: 81-3-5841-7825. Fax: 81-3-5841-8476. E-mail: htakaha{at}imcbns.iam.u-tokyo.ac.jp.

Journal of Bacteriology, August 2000, p. 4628-4631, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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