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Journal of Bacteriology, August 2000, p. 4661-4666, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Sequencing, Cloning, and High-Level Expression of the pfp Gene, Encoding a PPi-Dependent Phosphofructokinase from the Extremely Thermophilic Eubacterium Dictyoglomus thermophilum

Yan-Huai R. Ding,* Ron S. Ronimus, and Hugh W. Morgan

Thermophile Research Unit, Department of Biological Sciences, The University of Waikato, Hamilton, New Zealand

Received 21 March 2000/Accepted 26 May 2000

The sequencing, cloning, and expression of the pfp gene from Dictyoglomus thermophilum, which consists of 1,041 bp and encodes a pyrophosphate-dependent phosphofructokinase, are described. A phylogenetic analysis indicates that the enzyme is closely related to the pyrophosphate-dependent enzyme from Thermoproteus tenax. The recombinant and native enzymes share a high degree of similarity for most properties examined.

* Corresponding author. Mailing address: Thermophile Research Unit, The University of Waikato, Private Bag 3105, Hamilton, New Zealand. Phone: 0064-7-8388266. Fax: 0064-7-8384324. E-mail: yrd2{at}waikato.ac.nz or yrd6{at}hotmail.com.

Journal of Bacteriology, August 2000, p. 4661-4666, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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