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Journal of Bacteriology, August 2000, p. 4670-4672, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
H-NS-Dependent Regulation of Flagellar Synthesis Is
Mediated by a LysR Family Protein
Minsu
Ko and
Chankyu
Park*
National Creative Research Initiative Center
for Behavioral Genetics, Department of Biological Sciences, Korea
Advanced Institute of Science and Technology, Yusong-Ku, Taejon
305-701, Republic of Korea
Received 6 April 2000/Accepted 25 May 2000
H-NS regulates the flagellar master operon (flhDC) and
thus is necessary for flagellation of Escherichia coli.
However, the molecular mechanism of its regulation has remained
unknown. Genetic screening of a transposon insertion abolishing the
H-NS effect revealed a previously unidentified gene, named
hdfR, encoding a LysR family protein. Binding of purified
HdfR to the flhDC promoter was demonstrated by a DNA
mobility shift assay, indicating that HdfR is a transcriptional
regulator for the flagellar master operon. Furthermore, the expression
of the hdfR gene was shown to be negatively regulated by
H-NS.
*
Corresponding author. Mailing address: National
Creative Research Initiative Center for Behavioral Genetics, Department
of Biological Sciences, Korea Advanced Institute of Science and
Technology, Yusong-Ku, Taejon 305-701, Republic of Korea. Phone:
82-42-869-2629. Fax: 82-42-869-2610. E-mail:
ckpark{at}mail.kaist.ac.kr.
Journal of Bacteriology, August 2000, p. 4670-4672, Vol. 182, No. 16
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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