Microarray-Based Identification of a Novel Streptococcus pneumoniae Regulon Controlled by an Autoinduced Peptide

doi:10.1128/JB.182.17.4696-4703.2000

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Journal of Bacteriology, September 2000, p. 4696-4703, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Microarray-Based Identification of a Novel Streptococcus pneumoniae Regulon Controlled by an Autoinduced Peptide

Antoine de Saizieu,¹ Christophe Gardès,¹ Nicholas Flint,¹ Christian Wagner,¹ Markus Kamber,¹ Timothy J. Mitchell,² Wolfgang Keck,¹ Kurt E. Amrein,¹ and Roland Lange¹^,^*

Hoffmann-La Roche Ltd., CH-4070 Basel, Switzerland,¹ and Infection and Immunity, University of Glasgow, Glasgow G12 8QQ, Scotland²

Received 15 March 2000/Accepted 1 June 2000

We have identified in the Streptococcus pneumoniae genome sequence a two-component system (TCS13, Blp [bacteriocin-like peptide])which is closely related to quorum-sensing systems regulatingcell density-dependent phenotypes such as the development of geneticcompetence or the production of antimicrobial peptides in lacticacid bacteria. In this study we present evidence that TCS13 isa peptide-sensing system that controls a regulon including genesencoding Blps. Downstream of the Blp TCS (BlpH R) we identifiedopen reading frames (blpAB) that have the potential to encodean ABC transporter that is homologous to the ComA/B export systemfor the competence-stimulating peptide ComC. The putative translationproduct of blpC, a small gene located downstream of blpAB, hasa leader peptide with a Gly-Gly motif. This leader peptide istypical of precursors processed by this family of transporters.Microarray-based expression profiling showed that a syntheticoligopeptide corresponding to the processed form of BlpC (BlpC*)induces a distinct set of 16 genes. The changes in the expressionprofile elicited by synthetic BlpC* depend on BlpH since insertionalinactivation of its corresponding gene abolishes differentialgene induction. Comparison of the promoter regions of the blpgenes disclosed a conserved sequence element formed by two imperfectdirect repeats upstream of extended $-$ 10 promoter elements. Wepropose that BlpH is the sensor for BlpC* and the conserved sequenceelement is a recognition sequence for the BlpR responseregulator.

^* Corresponding author. Mailing address: Hoffmann-La Roche Ltd., Grenzacherstrasse 124, Bldg. 70/4, CH-4070 Basel, Switzerland.Phone: 41 61 687 40 33. Fax: 41 61 688 27 29. E-mail: roland.lange{at}roche.com.

Journal of Bacteriology, September 2000, p. 4696-4703, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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