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Journal of Bacteriology, September 2000, p. 4696-4703, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Microarray-Based Identification of a Novel Streptococcus pneumoniae Regulon Controlled by an Autoinduced Peptide

Antoine de Saizieu,1 Christophe Gardès,1 Nicholas Flint,1 Christian Wagner,1 Markus Kamber,1 Timothy J. Mitchell,2 Wolfgang Keck,1 Kurt E. Amrein,1 and Roland Lange1,*

Hoffmann-La Roche Ltd., CH-4070 Basel, Switzerland,1 and Infection and Immunity, University of Glasgow, Glasgow G12 8QQ, Scotland2

Received 15 March 2000/Accepted 1 June 2000

We have identified in the Streptococcus pneumoniae genome sequence a two-component system (TCS13, Blp [bacteriocin-like peptide]) which is closely related to quorum-sensing systems regulating cell density-dependent phenotypes such as the development of genetic competence or the production of antimicrobial peptides in lactic acid bacteria. In this study we present evidence that TCS13 is a peptide-sensing system that controls a regulon including genes encoding Blps. Downstream of the Blp TCS (BlpH R) we identified open reading frames (blpAB) that have the potential to encode an ABC transporter that is homologous to the ComA/B export system for the competence-stimulating peptide ComC. The putative translation product of blpC, a small gene located downstream of blpAB, has a leader peptide with a Gly-Gly motif. This leader peptide is typical of precursors processed by this family of transporters. Microarray-based expression profiling showed that a synthetic oligopeptide corresponding to the processed form of BlpC (BlpC*) induces a distinct set of 16 genes. The changes in the expression profile elicited by synthetic BlpC* depend on BlpH since insertional inactivation of its corresponding gene abolishes differential gene induction. Comparison of the promoter regions of the blp genes disclosed a conserved sequence element formed by two imperfect direct repeats upstream of extended -10 promoter elements. We propose that BlpH is the sensor for BlpC* and the conserved sequence element is a recognition sequence for the BlpR response regulator.

* Corresponding author. Mailing address: Hoffmann-La Roche Ltd., Grenzacherstrasse 124, Bldg. 70/4, CH-4070 Basel, Switzerland. Phone: 41 61 687 40 33. Fax: 41 61 688 27 29. E-mail: roland.lange{at}roche.com.

Journal of Bacteriology, September 2000, p. 4696-4703, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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