This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Valdivieso, M-H.
Right arrow Articles by Popolo, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Valdivieso, M-H.
Right arrow Articles by Popolo, L.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 2000, p. 4752-4757, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Chitin Synthesis in a gas1 Mutant of Saccharomyces cerevisiae

M-Henar Valdivieso,1 Laura Ferrario,2 Marina Vai,2,dagger Angel Duran,1 and Laura Popolo2,*

Departamento de Microbiologia y Genética/Instituto de Microbiologia Bioquimica, Universidad de Salamanca/CSIC, Campus Miguel de Unamuno, 37007 Salamanca, Spain,1 and Dipartimento di Fisiologia e Biochimica Generali, Università degli Studi di Milano, 20133 Milano, Italy2

Received 20 March 2000/Accepted 6 June 2000

The existence of a compensatory mechanism in response to cell wall damage has been proposed in yeast cells. The increase of chitin accumulation is part of this response. In order to study the mechanism of the stress-related chitin synthesis, we tested chitin synthase I (CSI), CSII, and CSIII in vitro activities in the cell-wall-defective mutant gas1Delta . CSI activity increased twofold with respect to the control, a finding in agreement with an increase in the expression of the CHS1 gene. However, deletion of the CHS1 gene did not affect the phenotype of the gas1Delta mutant and only slightly reduced the chitin content. Interestingly, in chs1 gas1 double mutants the lysed-bud phenotype, typical of chs1 null mutant, was suppressed, although in gas1 cells there was no reduction in chitinase activity. CHS3 expression was not affected in the gas1 mutant. Deletion of the CHS3 gene severely compromised the phenotype of gas1 cells, despite the fact that CSIII activity, assayed in membrane fractions, did not change. Furthermore, in chs3 gas1 cells the chitin level was about 10% that of gas1 cells. Thus, CSIII is the enzyme responsible for the hyperaccumulation of chitin in response to cell wall stress. However, the level of enzyme or the in vitro CSIII activity does not change. This result suggests that an interaction with a regulatory molecule or a posttranslational modification, which is not preserved during membrane fractionation, could be essential in vivo for the stress-induced synthesis of chitin.

* Corresponding author. Mailing address: Università degli Studi di Milano, Dipartimento di Fisiologia e Biochimica Generali, Via Celoria 26, 20133 Milano, Italy. Phone: 39(02)70644808. Fax: 39(02)70632811. E-mail: Laura.Popolo{at}unimi.it.

dagger Present address: Università degli Studi di Milano-Bicocca, Dipartimento di Biotecnologie e Bioscienze, 20126 Milano, Italy.

Journal of Bacteriology, September 2000, p. 4752-4757, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Popolo, L., Ragni, E., Carotti, C., Palomares, O., Aardema, R., Back, J. W., Dekker, H. L., de Koning, L. J., de Jong, L., de Koster, C. G. (2008). Disulfide Bond Structure and Domain Organization of Yeast {beta}(1,3)-Glucanosyltransferases Involved in Cell Wall Biogenesis. J. Biol. Chem. 283: 18553-18565 [Abstract] [Full Text]  
  • Kajiwara, K., Watanabe, R., Pichler, H., Ihara, K., Murakami, S., Riezman, H., Funato, K. (2008). Yeast ARV1 Is Required for Efficient Delivery of an Early GPI Intermediate to the First Mannosyltransferase during GPI Assembly and Controls Lipid Flow from the Endoplasmic Reticulum. Mol. Biol. Cell 19: 2069-2082 [Abstract] [Full Text]  
  • Grabinska, K. A., Magnelli, P., Robbins, P. W. (2007). Prenylation of Saccharomyces cerevisiae Chs4p Affects Chitin Synthase III Activity and Chitin Chain Length. Eukaryot Cell 6: 328-336 [Abstract] [Full Text]  
  • Perlinska-Lenart, U., Orlowski, J., Laudy, A. E., Zdebska, E., Palamarczyk, G., Kruszewska, J. S. (2006). Glycoprotein Hypersecretion Alters the Cell Wall in Trichoderma reesei Strains Expressing the Saccharomyces cerevisiae Dolichylphosphate Mannose Synthase Gene. Appl. Environ. Microbiol. 72: 7778-7784 [Abstract] [Full Text]  
  • Calzari, L., Orlandi, I., Alberghina, L., Vai, M. (2006). The Histone Deubiquitinating Enzyme Ubp10 Is Involved in rDNA Locus Control in Saccharomyces cerevisiae by Affecting Sir2p Association. Genetics 174: 2249-2254 [Abstract] [Full Text]  
  • Smits, G. J., Schenkman, L. R., Brul, S., Pringle, J. R., Klis, F. M. (2006). Role of Cell Cycle-regulated Expression in the Localized Incorporation of Cell Wall Proteins in Yeast. Mol. Biol. Cell 17: 3267-3280 [Abstract] [Full Text]  
  • Chasse, S. A., Flanary, P., Parnell, S. C., Hao, N., Cha, J. Y., Siderovski, D. P., Dohlman, H. G. (2006). Genome-Scale Analysis Reveals Sst2 as the Principal Regulator of Mating Pheromone Signaling in the Yeast Saccharomyces cerevisiae. Eukaryot Cell 5: 330-346 [Abstract] [Full Text]  
  • Zhong, Q., Gvozdenovic-Jeremic, J., Webster, P., Zhou, J., Greenberg, M. L. (2005). Loss of Function of KRE5 Suppresses Temperature Sensitivity of Mutants Lacking Mitochondrial Anionic Lipids. Mol. Biol. Cell 16: 665-675 [Abstract] [Full Text]  
  • Sestak, S., Hagen, I., Tanner, W., Strahl, S. (2004). Scw10p, a cell-wall glucanase/transglucosidase important for cell-wall stability in Saccharomyces cerevisiae. Microbiology 150: 3197-3208 [Abstract] [Full Text]  
  • Ram, A. F. J., Arentshorst, M., Damveld, R. A., vanKuyk, P. A., Klis, F. M., van den Hondel, C. A. M. J. J. (2004). The cell wall stress response in Aspergillus niger involves increased expression of the glutamine : fructose-6-phosphate amidotransferase-encoding gene (gfaA) and increased deposition of chitin in the cell wall. Microbiology 150: 3315-3326 [Abstract] [Full Text]  
  • Lesage, G., Sdicu, A.-M., Menard, P., Shapiro, J., Hussein, S., Bussey, H. (2004). Analysis of {beta}-1,3-Glucan Assembly in Saccharomyces cerevisiae Using a Synthetic Interaction Network and Altered Sensitivity to Caspofungin. Genetics 167: 35-49 [Abstract] [Full Text]  
  • Garcia, R., Bermejo, C., Grau, C., Perez, R., Rodriguez-Pena, J. M., Francois, J., Nombela, C., Arroyo, J. (2004). The Global Transcriptional Response to Transient Cell Wall Damage in Saccharomyces cerevisiae and Its Regulation by the Cell Integrity Signaling Pathway. J. Biol. Chem. 279: 15183-15195 [Abstract] [Full Text]  
  • Shiflett, S. L., Ward, D. M., Huynh, D., Vaughn, M. B, Simmons, J. C., Kaplan, J. (2004). Characterization of Vta1p, a Class E Vps Protein in Saccharomyces cerevisiae. J. Biol. Chem. 279: 10982-10990 [Abstract] [Full Text]  
  • Jones, D. L., Petty, J., Hoyle, D. C., Hayes, A., Ragni, E., Popolo, L., Oliver, S. G., Stateva, L. I. (2003). Transcriptome profiling of a Saccharomyces cerevisiae mutant with a constitutively activated Ras/cAMP pathway. Physiol. Genomics 16: 107-118 [Abstract] [Full Text]  
  • Bulik, D. A., Olczak, M., Lucero, H. A., Osmond, B. C., Robbins, P. W., Specht, C. A. (2003). Chitin Synthesis in Saccharomyces cerevisiae in Response to Supplementation of Growth Medium with Glucosamine and Cell Wall Stress. Eukaryot Cell 2: 886-900 [Abstract] [Full Text]  
  • Valdivia, R. H., Schekman, R. (2003). The yeasts Rho1p and Pkc1p regulate the transport of chitin synthase III (Chs3p) from internal stores to the plasma membrane. Proc. Natl. Acad. Sci. USA 100: 10287-10292 [Abstract] [Full Text]  
  • Wang, Q., Liu, H., Szaniszlo, P. J. (2002). Compensatory expression of five chitin synthase genes, a response to stress stimuli, in Wangiella (Exophiala) dermatitidis, a melanized fungal pathogen of humans. Microbiology 148: 2811-2817 [Abstract] [Full Text]  
  • Smits, G. J., van den Ende, H., Klis, F. M. (2001). Differential regulation of cell wall biogenesis during growth and development in yeast. Microbiology 147: 781-794 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»