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Journal of Bacteriology, September 2000, p. 4758-4763, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Two-Dimensional Protein Gel Electrophoresis Study of the Heat Stress Response of Bacillus subtilis Cells during Sporulation

Sara Movahedi and William Waites*

Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Loughborough LE12 5RD, United Kingdom

Received 13 March 2000/Accepted 7 June 2000

The heat resistance of spores of Bacillus subtilis formed at 30°C was enhanced by pretreatment at 48°C for 30 min, 60 min into sporulation, for all four strains examined. High-resolution two-dimensional gel electrophoresis showed the generation and/or overexpression of 60 proteins, 11 of which were specific to heat shock, concurrent to this acquired thermotolerance. The greatest number of new proteins was observed between 30 and 60 min after heat shock, and the longer the time between exponential growth and heat treatment, the fewer differences were observed on corresponding protein profiles. The time at which heating produced the maximum increase in spore resistance and the most new proteins on two-dimensional gels occurred before alkaline phosphatase and dipicolinic acid production and corresponded to stage I or II of sporulation. The stress proteins formed disappeared later in sporulation, suggesting that heat shock proteins increase spore heat resistance by altering spore structure rather than by repairing heat damage during germination and outgrowth.

* Corresponding author. Mailing address: Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Loughborough LE12 5RD, United Kingdom. Phone: 0115 9516160. Fax: 0115 9516162. E-mail: william.waites{at}nottingham.ac.uk.

Journal of Bacteriology, September 2000, p. 4758-4763, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Movahedi, S., Waites, W. (2002). Cold Shock Response in Sporulating Bacillus subtilis and Its Effect on Spore Heat Resistance. J. Bacteriol. 184: 5275-5281 [Abstract] [Full Text]  
  • Periago, P. M., van Schaik, W., Abee, T., Wouters, J. A. (2002). Identification of Proteins Involved in the Heat Stress Response of Bacillus cereus ATCC 14579. Appl. Environ. Microbiol. 68: 3486-3495 [Abstract] [Full Text]  
  • Melly, E., Setlow, P. (2001). Heat Shock Proteins Do Not Influence Wet Heat Resistance of Bacillus subtilis Spores. J. Bacteriol. 183: 779-784 [Abstract] [Full Text]  


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