This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kjærgaard, K.
Right arrow Articles by Klemm, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kjærgaard, K.
Right arrow Articles by Klemm, P.

 Previous Article  |  Next Article 

Journal of Bacteriology, September 2000, p. 4789-4796, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Antigen 43 from Escherichia coli Induces Inter- and Intraspecies Cell Aggregation and Changes in Colony Morphology of Pseudomonas fluorescens

Kristian Kjærgaard, Mark A. Schembri, Henrik Hasman, and Per Klemm*

Department of Microbiology, Technical University of Denmark, DK-2800 Lyngby, Denmark

Received 11 May 2000/Accepted 14 June 2000

Antigen 43 (Ag43) is a surface-displayed autotransporter protein of Escherichia coli. By virtue of its self-association characteristics, this protein is able to mediate autoaggregation and flocculation of E. coli cells in static cultures. Additionally, surface display of Ag43 is associated with a distinct frizzy colony morphology in E. coli. Here we show that Ag43 can be expressed in a functional form on the surface of the environmentally important Pseudomonas fluorescens strain SBW25 with ensuing cell aggregation and frizzy colony types. Using green fluorescence protein-tagged cells, we demonstrate that Ag43 can be used as a tool to provide interspecies cell aggregation between E. coli and P. fluorescens. Furthermore, Ag43 expression enhances biofilm formation in P. fluorescens to glass surfaces. The versatility of this protein was also reflected in Ag43 surface display in a variety of other gram-negative bacteria. Display of heterologous Ag43 in selected bacteria might offer opportunities for rational design of multispecies consortia where the concerted action of several bacterial species is required, e.g., waste treatment and degradation of pollutants.

* Corresponding author. Mailing address: Department of Microbiology, Bldg. 301, Technical University of Denmark, DK-2800 Lyngby, Denmark. Phone: 45 45 25 25 06. Fax: 45 45 93 28 09. E-mail: impk{at}pop.dtu.dk.

Journal of Bacteriology, September 2000, p. 4789-4796, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Vejborg, R. M., Klemm, P. (2009). Cellular chain formation in Escherichia coli biofilms. Microbiology 155: 1407-1417 [Abstract] [Full Text]  
  • Ulett, G. C., Mabbett, A. N., Fung, K. C., Webb, R. I., Schembri, M. A. (2007). The role of F9 fimbriae of uropathogenic Escherichia coli in biofilm formation. Microbiology 153: 2321-2331 [Abstract] [Full Text]  
  • Ulett, G. C., Valle, J., Beloin, C., Sherlock, O., Ghigo, J.-M., Schembri, M. A. (2007). Functional Analysis of Antigen 43 in Uropathogenic Escherichia coli Reveals a Role in Long-Term Persistence in the Urinary Tract. Infect. Immun. 75: 3233-3244 [Abstract] [Full Text]  
  • Fexby, S., Bjarnsholt, T., Jensen, P. O., Roos, V., Hoiby, N., Givskov, M., Klemm, P. (2007). Biological Trojan Horse: Antigen 43 Provides Specific Bacterial Uptake and Survival in Human Neutrophils. Infect. Immun. 75: 30-34 [Abstract] [Full Text]  
  • Ulett, G. C., Webb, R. I., Schembri, M. A. (2006). Antigen-43-mediated autoaggregation impairs motility in Escherichia coli. Microbiology 152: 2101-2110 [Abstract] [Full Text]  
  • Sherlock, O., Dobrindt, U., Jensen, J. B., Munk Vejborg, R., Klemm, P. (2006). Glycosylation of the Self-Recognizing Escherichia coli Ag43 Autotransporter Protein.. J. Bacteriol. 188: 1798-1807 [Abstract] [Full Text]  
  • Beloin, C., Michaelis, K., Lindner, K., Landini, P., Hacker, J., Ghigo, J.-M., Dobrindt, U. (2006). The Transcriptional Antiterminator RfaH Represses Biofilm Formation in Escherichia coli. J. Bacteriol. 188: 1316-1331 [Abstract] [Full Text]  
  • Sherlock, O., Vejborg, R. M., Klemm, P. (2005). The TibA Adhesin/Invasin from Enterotoxigenic Escherichia coli Is Self Recognizing and Induces Bacterial Aggregation and Biofilm Formation. Infect. Immun. 73: 1954-1963 [Abstract] [Full Text]  
  • Henderson, I. R., Navarro-Garcia, F., Desvaux, M., Fernandez, R. C., Ala'Aldeen, D. (2004). Type V Protein Secretion Pathway: the Autotransporter Story. Microbiol. Mol. Biol. Rev. 68: 692-744 [Abstract] [Full Text]  
  • Sherlock, O., Schembri, M. A., Reisner, A., Klemm, P. (2004). Novel Roles for the AIDA Adhesin from Diarrheagenic Escherichia coli: Cell Aggregation and Biofilm Formation. J. Bacteriol. 186: 8058-8065 [Abstract] [Full Text]  
  • Schembri, M. A., Dalsgaard, D., Klemm, P. (2004). Capsule Shields the Function of Short Bacterial Adhesins. J. Bacteriol. 186: 1249-1257 [Abstract] [Full Text]  
  • Veiga, E., de Lorenzo, V., Fernandez, L. A. (2003). Autotransporters as Scaffolds for Novel Bacterial Adhesins: Surface Properties of Escherichia coli Cells Displaying Jun/Fos Dimerization Domains. J. Bacteriol. 185: 5585-5590 [Abstract] [Full Text]  
  • Schembri, M. A., Hjerrild, L., Gjermansen, M., Klemm, P. (2003). Differential Expression of the Escherichiacoli Autoaggregation Factor Antigen 43. J. Bacteriol. 185: 2236-2242 [Abstract] [Full Text]  
  • Kjaergaard, K., Hasman, H., Schembri, M. A., Klemm, P. (2002). Antigen 43-Mediated Autotransporter Display, a Versatile Bacterial Cell Surface Presentation System. J. Bacteriol. 184: 4197-4204 [Abstract] [Full Text]  
  • Gabig, M., Herman-Antosiewicz, A., Kwiatkowska, M., Los, M., Thomas, M. S., Wegrzyn, G. (2002). The cell surface protein Ag43 facilitates phage infection of Escherichia coli in the presence of bile salts and carbohydrates. Microbiology 148: 1533-1542 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»