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Journal of Bacteriology, September 2000, p. 4841-4848, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Relative Roles of the fla/che PA, PD-3, and PsigD Promoters in Regulating Motility and sigD Expression in Bacillus subtilis

Joyce T. West,dagger William Estacio,Dagger and Leticia Márquez-Magaña*

Department of Biology, San Francisco State University, San Francisco, California 94132

Received 20 March 2000/Accepted 5 June 2000

Three promoters have been identified as having potentially important regulatory roles in governing expression of the fla/che operon and of sigD, a gene that lies near the 3' end of the operon. Two of these promoters, fla/che PA and PD-3, lie upstream of the >26-kb fla/che operon. The third promoter, PsigD, lies within the operon, immediately upstream of sigD. fla/che PA, transcribed by Esigma A, lies >= 24 kb upstream of sigD and appears to be largely responsible for sigD expression. PD-3, transcribed by Esigma D, has been proposed to participate in an autoregulatory positive feedback loop. PsigD, a minor sigma A-dependent promoter, has been implicated as essential for normal expression of the fla/che operon. We tested the proposed functions of these promoters in experiments that utilized strains that bear chromosomal deletions of fla/che PA, PD-3, or PsigD. Our analysis of these strains indicates that fla/che PA is absolutely essential for motility, that PD-3 does not function in positive feedback regulation of sigD expression, and that PsigD is not essential for normal fla/che expression. Further, our results suggest that an additional promoter(s) contributes to sigD expression.

* Corresponding author. Mailing address: Department of Biology, San Francisco State University, 1600 Holloway Ave., San Francisco, CA, 94132. Phone: (415) 338-3289. Fax: (415) 338-0927. E-mail: marquez{at}sfsu.edu.

dagger Present address: Department of Stomatology, University of California at San Francisco, San Francisco, CA 94143.

Dagger Present address: DNAX Research Institute, Palo Alto, CA 94304.

Journal of Bacteriology, September 2000, p. 4841-4848, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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