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Journal of Bacteriology, September 2000, p. 4998-5000, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Plasmid pGS5 from the Hyperthermophilic Archaeon Archaeoglobus profundus Is Negatively Supercoiled

Purificación López-García,1,2,* Patrick Forterre,1 John van der Oost,3 and Gaël Erauso1,3,4

Institut de Génétique et Microbiologie, Université Paris-Sud, 91405 Orsay Cedex,1 and Institut Universitaire Européen de la Mer, Université de Bretagne Occidental, 29280 Plouzané,4 France; División de Microbiología, Universidad Miguel Hernández, 03550 San Juan de Alicante Spain2; and Laboratory of Microbiology, Department of Biomolecular Sciences, Wageningen University, NL-6703 CT Wageningen, The Netherlands3

Received 24 January 2000/Accepted 26 May 2000

We present evidence that, in contrast to plasmids from other hyperthermophilic archaea, which are in the relaxed to positively supercoiled state, plasmid pGS5 (2.8 kb) from Archaeoglobus profundus is negatively supercoiled. This might be due to the presence of a gyrase introducing negative supercoils, since gyrase genes are present in the genome of its close relative A. fulgidus, and suggests that gyrase activity predominates over reverse gyrase whenever the two topoisomerases coexist in cells.

* Corresponding author. Mailing address: División de Microbiología, Facultad de Medicina, Universidad Miguel Hernández, Campus de San Juan, 03550 San Juan de Alicante, Spain. Phone: (34) 965 91 93 13. Fax: (34) 965 91 94 57. E-mail: puri{at}umh.es.

Journal of Bacteriology, September 2000, p. 4998-5000, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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