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Journal of Bacteriology, September 2000, p. 5005-5008, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Substrate Ambiguity of 3-Deoxy-D-manno-Octulosonate 8-Phosphate Synthase from Neisseria gonorrhoeae Revisited

Galina Ya Sheflyan, Appavu K. Sundaram, William P. Taylor, and Ronald W. Woodard*

Interdepartmental Program in Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109-1065

Received 22 March 2000/Accepted 25 May 2000

Homogeneous, recombinant 3-deoxy-D-manno-octulosonate 8-phosphate synthase from Neisseria gonorrhoeae is shown to catalyze the formation of 3-deoxy-D-manno-octulosonate 8-phosphate from phosphoenolpyruvate and D-arabinose 5-phosphate as determined from 1H-nuclear magnetic resonance analysis of the product. This enzyme does not catalyze the condensation of D-erythrose 4-phosphate and phosphoenolpyruvate to form 3-deoxy-D-ribo-heptulosonate 7-phosphate, as was previously reported (P. S. Subramaniam, G. Xie, T. Xia, and R. A. Jensen, J. Bacteriol. 180:119-127, 1998).

* Corresponding author. Mailing address: College of Pharmacy, 428 Church St., Ann Arbor, MI 48109-1065. Phone: (734) 764-7366. Fax: (734) 763-5633. E-mail: rww{at}umich.edu.

Journal of Bacteriology, September 2000, p. 5005-5008, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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