This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhao, J.
Right arrow Articles by Winkler, M. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhao, J.
Right arrow Articles by Winkler, M. E.

 Previous Article

Journal of Bacteriology, September 2000, p. 5025-5028, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Reduction of GC right-arrow  TA Transversion Mutation by Overexpression of MutS in Escherichia coli K-12

Jingyong Zhao and Malcolm E. Winkler*

Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, Texas 77030

Received 10 January 2000/Accepted 5 June 2000

Overexpression of the MutS repair protein significantly decreased the rate of lacZ GC right-arrow TA transversion mutation in stationary-phase and exponentially growing bacteria and in mutY and mutM mutants, which accumulate mismatches between 8-oxoguanine (8-oxoG) and adenine residues in DNA. Conversely, GC right-arrow TA transversion increased in mutL or mutS mutants in stationary phase. In contrast, overexpression of MutS did not appreciably reduce lacZ AT right-arrow CG transversion mutation in a mutT mutant. These results suggest that MutS-dependent repair can correct 8-oxoG:A mismatches in Escherichia coli cells but may not be able to compete with mutation fixation by MutY in mutT mutants.


* Corresponding author. Mailing address: Lilly Research Laboratories, Drop code 1543, Indianapolis, IN 46285. Phone: (317) 433-0095. Fax: (317) 276-9159. E-mail: Winkler_Malcolm_E{at}Lilly.com.


Journal of Bacteriology, September 2000, p. 5025-5028, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Polosina, Y. Y., Mui, J., Pitsikas, P., Cupples, C. G. (2009). The Escherichia coli Mismatch Repair Protein MutL Recruits the Vsr and MutH Endonucleases in Response to DNA Damage. J. Bacteriol. 191: 4041-4043 [Abstract] [Full Text]  
  • Tarassova, K., Tegova, R., Tover, A., Teras, R., Tark, M., Saumaa, S., Kivisaar, M. (2009). Elevated Mutation Frequency in Surviving Populations of Carbon-Starved rpoS-Deficient Pseudomonas putida Is Caused by Reduced Expression of Superoxide Dismutase and Catalase. J. Bacteriol. 191: 3604-3614 [Abstract] [Full Text]  
  • Russo, M. T., De Luca, G., Casorelli, I., Degan, P., Molatore, S., Barone, F., Mazzei, F., Pannellini, T., Musiani, P., Bignami, M. (2009). Role of MUTYH and MSH2 in the Control of Oxidative DNA Damage, Genetic Instability, and Tumorigenesis. Cancer Res. 69: 4372-4379 [Abstract] [Full Text]  
  • Herve-Jimenez, L., Guillouard, I., Guedon, E., Boudebbouze, S., Hols, P., Monnet, V., Maguin, E., Rul, F. (2009). Postgenomic Analysis of Streptococcus thermophilus Cocultivated in Milk with Lactobacillus delbrueckii subsp. bulgaricus: Involvement of Nitrogen, Purine, and Iron Metabolism. Appl. Environ. Microbiol. 75: 2062-2073 [Abstract] [Full Text]  
  • Bai, H., Lu, A-L. (2007). Physical and Functional Interactions between Escherichia coli MutY Glycosylase and Mismatch Repair Protein MutS. J. Bacteriol. 189: 902-910 [Abstract] [Full Text]  
  • Hall, L. M. C., Henderson-Begg, S. K. (2006). Hypermutable bacteria isolated from humans - a critical analysis.. Microbiology 152: 2505-2514 [Abstract] [Full Text]  
  • Davidsen, T., Bjoras, M., Seeberg, E. C., Tonjum, T. (2005). Antimutator Role of DNA Glycosylase MutY in Pathogenic Neisseria Species. J. Bacteriol. 187: 2801-2809 [Abstract] [Full Text]  
  • Meier, P., Wackernagel, W. (2005). Impact of mutS Inactivation on Foreign DNA Acquisition by Natural Transformation in Pseudomonas stutzeri. J. Bacteriol. 187: 143-154 [Abstract] [Full Text]  
  • Lee, C.-Y., Bai, H., Houle, R., Wilson, G. M., Lu, A-L. (2004). An Escherichia coli MutY Mutant without the Six-helix Barrel Domain Is a Dimer in Solution and Assembles Cooperatively into Multisubunit Complexes with DNA. J. Biol. Chem. 279: 52653-52663 [Abstract] [Full Text]  
  • Li, L., Lu, A-L. (2003). The C-terminal domain of Escherichia coli MutY is involved in DNA binding and glycosylase activities. Nucleic Acids Res 31: 3038-3049 [Abstract] [Full Text]  
  • Li, B., Tsui, H.-C. T., LeClerc, J. E., Dey, M., Winkler, M. E., Cebula, T. A. (2003). Molecular analysis of mutS expression and mutation in natural isolates of pathogenic Escherichia coli. Microbiology 149: 1323-1331 [Abstract] [Full Text]  
  • Wyrzykowski, J., Volkert, M. R. (2003). The Escherichia coli Methyl-Directed Mismatch Repair System Repairs Base Pairs Containing Oxidative Lesions. J. Bacteriol. 185: 1701-1704 [Abstract] [Full Text]  
  • Saumaa, S., Tover, A., Kasak, L., Kivisaar, M. (2002). Different Spectra of Stationary-Phase Mutations in Early-Arising versus Late-Arising Mutants of Pseudomonas putida: Involvement of the DNA Repair Enzyme MutY and the Stationary-Phase Sigma Factor RpoS. J. Bacteriol. 184: 6957-6965 [Abstract] [Full Text]  
  • Young, D. M., Ornston, L. N. (2001). Functions of the Mismatch Repair Gene mutS from Acinetobacter sp. Strain ADP1. J. Bacteriol. 183: 6822-6831 [Abstract] [Full Text]  
  • Kondo, A., Safaei, R., Mishima, M., Niedner, H., Lin, X., Howell, S. B. (2001). Hypoxia-induced Enrichment and Mutagenesis of Cells That Have Lost DNA Mismatch Repair. Cancer Res. 61: 7603-7607 [Abstract] [Full Text]  
  • Zhao, J., Leung, H.-C. E., Winkler, M. E. (2001). The miaA Mutator Phenotype of Escherichia coli K-12 Requires Recombination Functions. J. Bacteriol. 183: 1796-1800 [Abstract] [Full Text]  
  • Volkert, M. R., Elliott, N. A., Housman, D. E. (2000). Functional genomics reveals a family of eukaryotic oxidation protection genes. Proc. Natl. Acad. Sci. USA 10.1073/pnas.260495897v1 [Abstract] [Full Text]  
  • Volkert, M. R., Elliott, N. A., Housman, D. E. (2000). Functional genomics reveals a family of eukaryotic oxidation protection genes. Proc. Natl. Acad. Sci. USA 97: 14530-14535 [Abstract] [Full Text]