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Journal of Bacteriology, September 2000, p. 5025-5028, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Reduction of GC right-arrow  TA Transversion Mutation by Overexpression of MutS in Escherichia coli K-12

Jingyong Zhao and Malcolm E. Winkler*

Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, Texas 77030

Received 10 January 2000/Accepted 5 June 2000

Overexpression of the MutS repair protein significantly decreased the rate of lacZ GC right-arrow TA transversion mutation in stationary-phase and exponentially growing bacteria and in mutY and mutM mutants, which accumulate mismatches between 8-oxoguanine (8-oxoG) and adenine residues in DNA. Conversely, GC right-arrow TA transversion increased in mutL or mutS mutants in stationary phase. In contrast, overexpression of MutS did not appreciably reduce lacZ AT right-arrow CG transversion mutation in a mutT mutant. These results suggest that MutS-dependent repair can correct 8-oxoG:A mismatches in Escherichia coli cells but may not be able to compete with mutation fixation by MutY in mutT mutants.

* Corresponding author. Mailing address: Lilly Research Laboratories, Drop code 1543, Indianapolis, IN 46285. Phone: (317) 433-0095. Fax: (317) 276-9159. E-mail: Winkler_Malcolm_E{at}Lilly.com.

Journal of Bacteriology, September 2000, p. 5025-5028, Vol. 182, No. 17
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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