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Journal of Bacteriology, September 2000, p. 5167-5171, Vol. 182, No. 18
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Positive cis-Acting DNA Element Is Required for High-Level Transcription in Chlamydia

Chris S. Schaumburg1 and Ming Tan1,2,*

Department of Microbiology and Molecular Genetics1 and Department of Medicine,2 College of Medicine, University of California, Irvine, California 92697-4025

Received 10 April 2000/Accepted 18 June 2000

The spacer A/T region is a positive cis-acting DNA element that was identified in the Chlamydia trachomatis rRNA promoter region. We have now demonstrated that similar sequences in other chlamydial promoters are important for transcription. Substitution of candidate spacer A/T regions in four chlamydial promoters decreased transcription by partially purified C. trachomatis RNA polymerase in an in vitro transcription assay. Addition of a spacer A/T region to the dnaK promoter, which does not contain an identifiable spacer A/T region, increased transcription 16-fold. Transcription of Escherichia coli promoters by C. trachomatis RNA polymerase also appeared to be dependent on the spacer A/T region. However, the effect of the spacer A/T region on transcription by E. coli RNA polymerase was small. In summary, the spacer A/T region is a novel DNA element that is required for high-level transcription of many promoters by chlamydial RNA polymerase.

* Corresponding author. Mailing address: B234, Med Sci I, Department of Microbiology & Molecular Genetics, University of California, Irvine, CA 92697-4025. Phone: (949) 824-3397. Fax: (949) 824-8598. E-mail: mingt{at}uci.edu.

Journal of Bacteriology, September 2000, p. 5167-5171, Vol. 182, No. 18
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Niehus, E., Cheng, E., Tan, M. (2008). DNA Supercoiling-Dependent Gene Regulation in Chlamydia. J. Bacteriol. 190: 6419-6427 [Abstract] [Full Text]  
  • Schaumburg, C. S., Tan, M. (2006). Arginine-Dependent Gene Regulation via the ArgR Repressor Is Species Specific in Chlamydia. J. Bacteriol. 188: 919-927 [Abstract] [Full Text]  
  • Wilson, A. C., Tan, M. (2004). Stress Response Gene Regulation in Chlamydia Is Dependent on HrcA-CIRCE Interactions. J. Bacteriol. 186: 3384-3391 [Abstract] [Full Text]  
  • Schaumburg, C. S., Tan, M. (2003). Mutational analysis of the Chlamydia trachomatis dnaK promoter defines the optimal -35 promoter element. Nucleic Acids Res 31: 551-555 [Abstract] [Full Text]  
  • Wilson, A. C., Tan, M. (2002). Functional Analysis of the Heat Shock Regulator HrcA of Chlamydia trachomatis. J. Bacteriol. 184: 6566-6571 [Abstract] [Full Text]  


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