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Journal of Bacteriology, October 2000, p. 5563-5571, Vol. 182, No. 19
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Yersinia enterocolitica ClpB Affects Levels of Invasin and Motility

Julie L. Badger,1,dagger Briana M. Young,2,Dagger Andrew J. Darwin,2 and Virginia L. Miller1,2,3,*

Department of Microbiology and Molecular Genetics, University of California, Los Angeles, Los Angeles, California 90095,1 and Department of Molecular Microbiology2 and Division of Infectious Disease, Department of Pediatrics,3 Washington University School of Medicine and St. Louis Children's Hospital, St. Louis, Missouri 63110

Received 6 January 2000/Accepted 13 July 2000

Expression of the Yersinia enterocolitica inv gene is dependent on growth phase and temperature. inv is maximally expressed at 23°C in late-exponential- to early-stationary-phase cultures. We previously reported the isolation of a Y. enterocolitica mutant (JB1A8v) that shows a decrease in invasin levels yet is hypermotile when grown at 23°C. JB1A8v has a transposon insertion within uvrC. Described here is the isolation and characterization of a clone that suppresses these mutant phenotypes of the uvrC mutant JB1A8v. This suppressing clone encodes ClpB (a Clp ATPase homologue). The Y. enterocolitica ClpB homologue is 30 to 40% identical to the ClpB proteins from various bacteria but is 80% identical to one of the two ClpB homologues of Yersinia pestis. A clpB::TnMax2 insertion mutant (JB69Qv) was constructed and determined to be deficient in invasin production and nonmotile when grown at 23°C. Analysis of inv and fleB (flagellin gene) transcript levels in JB69Qv suggested that ClpB has both transcriptional and posttranscriptional effects. In contrast, a clpB null mutant, BY1v, had no effect on invasin levels or motility. A model accounting for these observations is presented.

* Corresponding author. Present address: Department of Molecular Microbiology, Campus Box 8208, Washington University School of Medicine, St. Louis, MO 63110-1093. Phone: (314) 286-2891. Fax: (314) 286-2896. E-mail: virginia{at}borcim.wustl.edu.

dagger Present address: Department of Pathology, University of Southern California Keck School of Medicine, Children's Hospital Los Angeles, Los Angeles, CA 90027.

Dagger Present address: Department of Food Science and Technology, University of California at Davis, Davis, CA 95616.

Journal of Bacteriology, October 2000, p. 5563-5571, Vol. 182, No. 19
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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