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Journal of Bacteriology, October 2000, p. 5596-5599, Vol. 182, No. 19
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Dual Transcriptional Regulation of the Escherichia coli Phosphate-Starvation-Inducible psiE Gene of the Phosphate Regulon by PhoB and the Cyclic AMP (cAMP)-cAMP Receptor Protein Complex

Soo-Ki Kim,1 Sigenobu Kimura,2 Hideo Shinagawa,2 Atsuo Nakata,3 Ki-Sung Lee,1 Barry L. Wanner,4 and Kozo Makino2,*

Research Center for Biomedicinal Resources (Bio-Med RRC), Paichai University, Taejon 302-735, Korea1; Department of Molecular Microbiology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871,2 and Department of Marine Biotechnology, Faculty of Technology, Fukuyama University, Fukuyama, Hiroshima 729-0292,3 Japan; and Department of Biological Sciences, Purdue University, West Lafayette, Indiana 479074

Received 10 March 2000/Accepted 7 July 2000

We have shown that the Escherichia coli phosphate-starvation-inducible psiE gene is regulated by both phosphate and the carbon source by using both lacZ and chloramphenicol acetyltransferase gene (cat) fusions. Yet, under all conditions tested, a single transcriptional start site lying 7 bp downstream of a predicted -10 region was revealed by primer extension analysis. DNase I footprinting showed that the PhoB transcriptional-activator protein protects two predicted pho boxes lying upstream of and near the -35 promoter region. Similar analysis showed that the cyclic AMP (cAMP)-cAMP receptor protein (cAMP-CRP) complex binds a region that overlaps with the downstream pho box. These results, together with measurements of the in vivo psiE promoter activity under various conditions, show that expression of the psiE gene is under direct positive and negative control by PhoB and cAMP-CRP, respectively.

* Corresponding author. Mailing address: Department of Molecular Microbiology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Phone: 81-6-6879-8318. Fax: 81-6-6879-8320. E-mail: makino{at}bkns01.biken.osaka-u.ac.jp.

Journal of Bacteriology, October 2000, p. 5596-5599, Vol. 182, No. 19
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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