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Journal of Bacteriology, October 2000, p. 5606-5610, Vol. 182, No. 19
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Action of the Thiamine Antagonist Bacimethrin on Thiamine Biosynthesis

Julie L. Zilles,dagger Laura R. Croal,Dagger and Diana M. Downs*

Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706

Received 17 April 2000/Accepted 17 July 2000

Bacimethrin is an analog of the 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) moiety of thiamine and inhibits the growth of Salmonella enterica serovar Typhimurium on a defined medium. Two classes of mutants that had increased bacimethrin resistance were isolated and characterized. Results showed that overexpression of the thi operon or specific lesions in thiD resulted in a bacimethrin-resistant phenotype. Phenotypic analyses of the thiD mutants suggested that they had a specific defect in one of the two kinase activities associated with this gene product and, further, that ThiD and not PdxK was primarily responsible for salvage of HMP from the medium.

* Corresponding author. Mailing address: Department of Bacteriology, University of Wisconsin-Madison, 1550 Linden Dr., Madison, WI 53706. Phone: (608) 265-4630. Fax: (608) 262-9865. E-mail: downs{at}bact.wisc.edu.

dagger Present address: Department of Civil and Environmental Engineering, University of Wisconsin-Madison, Madison, WI 53706.

Dagger Present address: California Institute of Technology, Pasadena, CA 91125.

Journal of Bacteriology, October 2000, p. 5606-5610, Vol. 182, No. 19
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Karunakaran, R., Ebert, K., Harvey, S., Leonard, M. E., Ramachandran, V., Poole, P. S. (2006). Thiamine Is Synthesized by a Salvage Pathway in Rhizobium leguminosarum bv. viciae Strain 3841.. J. Bacteriol. 188: 6661-6668 [Abstract] [Full Text]  
  • Lawhorn, B. G., Gerdes, S. Y., Begley, T. P. (2004). A Genetic Screen for the Identification of Thiamin Metabolic Genes. J. Biol. Chem. 279: 43555-43559 [Abstract] [Full Text]  


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