Journal of Bacteriology, October 2000, p. 5606-5610, Vol. 182, No. 19
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

andDepartment of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706
Received 17 April 2000/Accepted 17 July 2000
Bacimethrin is an analog of the 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) moiety of thiamine and inhibits the growth of Salmonella enterica serovar Typhimurium on a defined medium. Two classes of mutants that had increased bacimethrin resistance were isolated and characterized. Results showed that overexpression of the thi operon or specific lesions in thiD resulted in a bacimethrin-resistant phenotype. Phenotypic analyses of the thiD mutants suggested that they had a specific defect in one of the two kinase activities associated with this gene product and, further, that ThiD and not PdxK was primarily responsible for salvage of HMP from the medium.
Present address: Department of Civil and Environmental Engineering,
University of Wisconsin-Madison, Madison, WI 53706.
Present address: California Institute of Technology, Pasadena, CA 91125.
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