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Journal of Bacteriology, January 2000, p. 311-319, Vol. 182, No. 2
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The PspA Protein of Escherichia coli Is a Negative Regulator of ⁵⁴-Dependent Transcription

Jonathan Dworkin,^* Goran Jovanovic, and Peter Model

Laboratory of Genetics, The Rockefeller University, New York, New York 10021

Received 28 May 1999/Accepted 27 October 1999

In Eubacteria, expression of genes transcribed by an RNA polymerase holoenzyme containing the alternate sigma factor ⁵⁴ is positively regulated by proteins belonging to the family of enhancer-binding proteins (EBPs). These proteins bind to upstream activation sequences and are required for the initiation of transcription at the ⁵⁴-dependent promoters. They are typically inactive until modified in their N-terminal regulatory domain either by specific phosphorylation or by the binding of a small effector molecule. EBPs lacking this domain, such as the PspF activator of the ⁵⁴-dependent pspA promoter, are constitutively active. We describe here the in vivo and in vitro properties of the PspA protein of Escherichia coli, which negatively regulates expression of the pspA promoter without binding DNA directly.

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^* Corresponding author. Present address: Dept. of Molecular and Cellular Biology, Harvard University, 16 Divinity Ave., Cambridge, MA 02138. Phone: (617) 495-0532. Fax: (617) 496-4642. E-mail: dworkin2{at}fas.harvard.edu.

Present address: Department de Biochimie Medicale, Centre Medical Universitaire, 1211 Geneva 4, Switzerland.

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Journal of Bacteriology, January 2000, p. 311-319, Vol. 182, No. 2
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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