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Journal of Bacteriology, January 2000, p. 418-424, Vol. 182, No. 2
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification and Characterization of a New Prespore-Specific Regulatory Gene, rsfA, of Bacillus subtilis

Ling Juan Wu and Jeff Errington*

Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom

Received 11 August 1999/Accepted 27 October 1999

Differential gene expression during Bacillus subtilis sporulation is controlled by sigma factors and other regulatory effectors. The first compartmentalized sigma factor, sigma F, is active specifically in the prespore compartment. During our screening for new chromosome segregation mutants using a sigma F-dependent gpr-lacZ reporter as a probe, we identified a new gene (ywfN) required for maximal expression of the reporter and named it rsfA. The product of rsfA has features of gene regulatory proteins, and the protein colocalizes with DNA. The expression of rsfA is under the control of both sigma F and sigma G. Null mutations in rsfA have different effects on the expression of sigma F-dependent genes, suggesting that the RsfA protein is a regulator of transcription that fine-tunes gene expression in the prespore.

* Corresponding author. Mailing address: Sir William Dunn School of Pathology, University of Oxford, South Parks Rd., Oxford OX1 3RE, United Kingdom. Phone: (44) 1865 275561. Fax (44) 1865 275556. E-mail: erring{at}molbiol.ox.ac.uk.

Journal of Bacteriology, January 2000, p. 418-424, Vol. 182, No. 2
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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  • Amaya, E., Khvorova, A., Piggot, P. J. (2001). Analysis of Promoter Recognition In Vivo Directed by {sigma}F of Bacillus subtilis by Using Random-Sequence Oligonucleotides. J. Bacteriol. 183: 3623-3630 [Abstract] [Full Text]  
  • Setlow, B., McGinnis, K. A., Ragkousi, K., Setlow, P. (2000). Effects of Major Spore-Specific DNA Binding Proteins on Bacillus subtilis Sporulation and Spore Properties. J. Bacteriol. 182: 6906-6912 [Abstract] [Full Text]  


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