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Journal of Bacteriology, January 2000, p. 498-503, Vol. 182, No. 2
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The N-Terminal Region of the Escherichia coli WecA (Rfe) Protein, Containing Three Predicted Transmembrane Helices, Is Required for Function but Not for Membrane Insertion

Amal O. Amer1 and Miguel A. Valvano1,2,*

Department of Microbiology and Immunology1 and Department of Medicine,2 University of Western Ontario, London, Ontario N6A 5C1, Canada

Received 5 August 1999/Accepted 21 October 1999

The correct site for translation initiation for Escherichia coli WecA (Rfe), presumably involved in catalyzing the transfer of N-acetylglucosamine 1-phosphate to undecaprenylphosphate, was determined by using its FLAG-tagged derivatives. The N-terminal region containing three predicted transmembrane helices was found to be necessary for function but not for membrane localization of this protein.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada. Phone: (519) 661-3996. Fax: (519) 661-3499. E-mail: mvalvano{at}julian.uwo.ca.

Journal of Bacteriology, January 2000, p. 498-503, Vol. 182, No. 2
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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