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Journal of Bacteriology, January 2000, p. 513-517, Vol. 182, No. 2
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The Amino Terminus of Salmonella enterica Serovar Typhimurium sigma 54 Is Required for Interactions with an Enhancer-Binding Protein and Binding to Fork Junction DNA

Mary T. Kelly and Timothy R. Hoover*

Department of Microbiology, University of Georgia, Athens, Georgia 30602

Received 24 June 1999/Accepted 22 October 1999

Transcription initiation by the sigma 54-RNA polymerase holoenzyme requires an enhancer-binding protein that is thought to contact sigma 54 to activate transcription. To identify potential enhancer-binding protein contact sites in sigma 54, we compared the abilities of wild-type and truncated forms of Salmonella enterica serovar Typhimurium sigma 54 to interact with the enhancer-binding protein DctD in a chemical cross-linking assay. Removal of two regions in the amino-terminal portion of sigma 54, residues 57 to 105 and residues 144 to 179, prevented cross-linking, but removal of either region alone did not. In addition, deletion of 56 amino-terminal residues of sigma 54 (region I) reduced the affinity of the protein for a fork junction DNA probe.

* Corresponding author. Mailing address: Department of Microbiology, 527 Biological Sciences Building, University of Georgia, Athens, GA 30602. Phone: (706) 542-2675. Fax: (706) 542-2674. E-mail: trhoover{at}arches.uga.edu.

Journal of Bacteriology, January 2000, p. 513-517, Vol. 182, No. 2
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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