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Journal of Bacteriology, January 2000, p. 555-560, Vol. 182, No. 2
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The TRAP-Like SplA Protein Is a trans-Acting Negative Regulator of Spore Photoproduct Lyase Synthesis during Bacillus subtilis Sporulation

Patricia Fajardo-Cavazos and Wayne L. Nicholson*

Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721

Received 19 March 1999/Accepted 18 October 1999

UV resistance of bacterial endospores derives from a unique DNA photochemistry in which the major UV photoproduct is the thymine dimer 5-thyminyl-5,6-dihydrothymine (spore photoproduct [SP]) instead of cyclobutane pyrimidine dimers. Repair of SP during spore germination is due in large part to the activity of the enzyme SP lyase encoded by splB, the second cistron of the splAB operon. Expression of the splAB operon in Bacillus subtilis is transcriptionally activated by the Esigma G form of RNA polymerase during morphological stage III in the developing forespore compartment, and SP lyase is packaged into the dormant spore. In addition to temporal and compartmental control of splAB expression, a second regulatory circuit which modulates the level of expression of splB-lacZ fusions without altering their developmental timing or compartmentalization is reported here. This second regulatory circuit involves the negative action of the splA gene product, a 79-amino-acid protein with approximately 50% similarity and 17% identity to TRAP, the tryptophan RNA-binding attenuation protein from B. subtilis and Bacillus pumilus.

* Corresponding author. Mailing address: Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ 85721. Phone: (520) 621-2157. Fax (520) 621-6366. E-mail: wln{at}u.arizona.edu.

Journal of Bacteriology, January 2000, p. 555-560, Vol. 182, No. 2
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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