This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Wezel, G. P.
Right arrow Articles by Kraal, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Wezel, G. P.
Right arrow Articles by Kraal, B.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2000, p. 5653-5662, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

ssgA Is Essential for Sporulation of Streptomyces coelicolor A3(2) and Affects Hyphal Development by Stimulating Septum Formation

Gilles P. van Wezel,1,* Jannes van der Meulen,2 Shinichi Kawamoto,3 Ruud G. M. Luiten,4 Henk K. Koerten,2 and Barend Kraal1

Department of Biochemistry, Leiden Institute of Chemistry, Leiden University,1 and Center for Electron Microscopy, Leiden University Medical Center,2 2300 RA Leiden, and DSM Anti-Infectives, 2600 MA Delft,4 The Netherlands, and National Food Research Institute, Tsukuba City, Japan3

Received 31 March 2000/Accepted 26 June 2000

The role of ssgA in cell division and development of streptomycetes was analyzed. An ssgA null mutant of Streptomyces coelicolor produced aerial hyphae but failed to sporulate, and ssgA can therefore be regarded as a novel whi gene. In addition to the morphological changes, antibiotic production was also disturbed, with strongly reduced actinorhodin production. These defects could be complemented by plasmid-borne ssgA. In the wild-type strain, transcription of ssgA was induced by nutritional shift-down and was shown to be linked to that of the upstream-located gene ssgR, which belongs to the family of iclR-type transcriptional regulator genes. Analysis of mycelium harvested from liquid-grown cultures by transmission electron microscopy showed that septum formation had strongly increased in ssgA-overexpressing strains in comparison to wild-type S. coelicolor and that spore-like compartments were produced at high frequency. Furthermore, the hyphae were significantly wider and contained irregular and often extremely thick septa. These data underline the important role for ssgA in Streptomyces cell division.

* Corresponding author. Mailing address: Department of Biochemistry, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands. Phone: (31) 71 5274310. Fax: (31) 71 5274340. E-mail: g.wezel{at}chem.leidenuniv.nl.

Journal of Bacteriology, October 2000, p. 5653-5662, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Traag, B. A., Seghezzi, N., Vijgenboom, E., van Wezel, G. P. (2007). Characterization of the Sporulation Control Protein SsgA by Use of an Efficient Method To Create and Screen Random Mutant Libraries in Streptomycetes. Appl. Environ. Microbiol. 73: 2085-2092 [Abstract] [Full Text]  
  • van Wezel, G. P., Krabben, P., Traag, B. A., Keijser, B. J. F., Kerste, R., Vijgenboom, E., Heijnen, J. J., Kraal, B. (2006). Unlocking Streptomyces spp. for Use as Sustainable Industrial Production Platforms by Morphological Engineering. Appl. Environ. Microbiol. 72: 5283-5288 [Abstract] [Full Text]  
  • Zhu, D., He, X., Zhou, X., Deng, Z. (2005). Expression of the melC Operon in Several Streptomyces Strains Is Positively Regulated by AdpA, an AraC Family Transcriptional Regulator Involved in Morphological Development in Streptomyces coelicolor. J. Bacteriol. 187: 3180-3187 [Abstract] [Full Text]  
  • Grantcharova, N., Lustig, U., Flardh, K. (2005). Dynamics of FtsZ Assembly during Sporulation in Streptomyces coelicolor A3(2). J. Bacteriol. 187: 3227-3237 [Abstract] [Full Text]  
  • Yamazaki, H., Ohnishi, Y., Horinouchi, S. (2003). Transcriptional Switch On of ssgA by A-Factor, Which Is Essential for Spore Septum Formation in Streptomyces griseus. J. Bacteriol. 185: 1273-1283 [Abstract] [Full Text]  
  • Zhang, R.-g., Kim, Y., Skarina, T., Beasley, S., Laskowski, R., Arrowsmith, C., Edwards, A., Joachimiak, A., Savchenko, A. (2002). Crystal Structure of Thermotoga maritima 0065, a Member of the IclR Transcriptional Factor Family. J. Biol. Chem. 277: 19183-19190 [Abstract] [Full Text]  
  • Seo, J.-W., Ohnishi, Y., Hirata, A., Horinouchi, S. (2002). ATP-Binding Cassette Transport System Involved in Regulation of Morphological Differentiation in Response to Glucose in Streptomyces griseus. J. Bacteriol. 184: 91-103 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»