This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Parish, T.
Right arrow Articles by Stoker, N. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Parish, T.
Right arrow Articles by Stoker, N. G.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2000, p. 5715-5720, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

glnE Is an Essential Gene in Mycobacterium tuberculosis

Tanya Parish* and Neil G. Stoker

Department of Infectious & Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom

Received 9 June 2000/Accepted 25 July 2000

Mycobacterium tuberculosis possesses a homologue of glnE, potentially encoding a regulator of glutamine synthetase activity. We attempted to construct glnE-disrupted mutants using a two-step strategy, whereby a single-crossover strain was first isolated, followed by sacB counterselection to isolate the double-crossover strain. Of 192 sucrose-resistant colonies tested, none were mutants, although the wild-type double crossover could be easily isolated. When a second copy of the wild-type glnE was integrated into the chromosome, we could isolate both wild-type and mutant double-crossover strains. Thus, the chromosomal gene could only be replaced with a disrupted copy when another functional copy of the gene was provided, demonstrating that this gene is essential under the conditions tested.

* Corresponding author. Mailing address: Department of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London, UK WC1E 7HT. Phone: 44 (0)20-7927 2425. Fax: 44 (0)20-7637 4314. E-mail: Tanya.Parish{at}lshtm.ac.uk.

Journal of Bacteriology, October 2000, p. 5715-5720, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Porter, J. L., Tobias, N. J., Hong, H., Tuck, K. L., Jenkin, G. A., Stinear, T. P. (2009). Transfer, stable maintenance and expression of the mycolactone polyketide megasynthase mls genes in a recombination-impaired Mycobacterium marinum. Microbiology 155: 1923-1933 [Abstract] [Full Text]  
  • McLean, K. J., Carroll, P., Lewis, D. G., Dunford, A. J., Seward, H. E., Neeli, R., Cheesman, M. R., Marsollier, L., Douglas, P., Smith, W. E., Rosenkrands, I., Cole, S. T., Leys, D., Parish, T., Munro, A. W. (2008). Characterization of Active Site Structure in CYP121: A CYTOCHROME P450 ESSENTIAL FOR VIABILITY OF MYCOBACTERIUM TUBERCULOSIS H37Rv. J. Biol. Chem. 283: 33406-33416 [Abstract] [Full Text]  
  • Amon, J., Brau, T., Grimrath, A., Hanssler, E., Hasselt, K., Holler, M., Jessberger, N., Ott, L., Szokol, J., Titgemeyer, F., Burkovski, A. (2008). Nitrogen Control in Mycobacterium smegmatis: Nitrogen-Dependent Expression of Ammonium Transport and Assimilation Proteins Depends on the OmpR-Type Regulator GlnR. J. Bacteriol. 190: 7108-7116 [Abstract] [Full Text]  
  • Carroll, P., Pashley, C. A., Parish, T. (2008). Functional Analysis of GlnE, an Essential Adenylyl Transferase in Mycobacterium tuberculosis. J. Bacteriol. 190: 4894-4902 [Abstract] [Full Text]  
  • Eoh, H., Brown, A. C., Buetow, L., Hunter, W. N., Parish, T., Kaur, D., Brennan, P. J., Crick, D. C. (2007). Characterization of the Mycobacterium tuberculosis 4-Diphosphocytidyl-2-C-Methyl-D-Erythritol Synthase: Potential for Drug Development. J. Bacteriol. 189: 8922-8927 [Abstract] [Full Text]  
  • Lee, S., Jeon, B.-Y., Bardarov, S., Chen, M., Morris, S. L., Jacobs, W. R. Jr. (2006). Protection Elicited by Two Glutamine Auxotrophs of Mycobacterium tuberculosis and In Vivo Growth Phenotypes of the Four Unique Glutamine Synthetase Mutants in a Murine Model.. Infect. Immun. 74: 6491-6495 [Abstract] [Full Text]  
  • Pashley, C. A., Brown, A. C., Robertson, D., Parish, T. (2006). Identification of the Mycobacterium tuberculosis GlnE promoter and its response to nitrogen availability.. Microbiology 152: 2727-2734 [Abstract] [Full Text]  
  • Carroll, P., Muttucumaru, D. G. N., Parish, T. (2005). Use of a Tetracycline-Inducible System for Conditional Expression in Mycobacterium tuberculosis and Mycobacterium smegmatis. Appl. Environ. Microbiol. 71: 3077-3084 [Abstract] [Full Text]  
  • Mehta, R., Pearson, J. T., Mahajan, S., Nath, A., Hickey, M. J., Sherman, D. R., Atkins, W. M. (2004). Adenylylation and Catalytic Properties of Mycobacterium tuberculosis Glutamine Synthetase Expressed in Escherichia coli versus Mycobacteria. J. Biol. Chem. 279: 22477-22482 [Abstract] [Full Text]  
  • Sareen, D., Newton, G. L., Fahey, R. C., Buchmeier, N. A. (2003). Mycothiol Is Essential for Growth of Mycobacterium tuberculosis Erdman. J. Bacteriol. 185: 6736-6740 [Abstract] [Full Text]  
  • Collins, D. M., Wilson, T., Campbell, S., Buddle, B. M., Wards, B. J., Hotter, G., de Lisle, G. W. (2002). Production of avirulent mutants of Mycobacterium bovis with vaccine properties by the use of illegitimate recombination and screening of stationary-phase cultures. Microbiology 148: 3019-3027 [Abstract] [Full Text]  
  • Parish, T., Stoker, N. G. (2002). The common aromatic amino acid biosynthesis pathway is essential in Mycobacterium tuberculosis. Microbiology 148: 3069-3077 [Abstract] [Full Text]  
  • Rodriguez, G. M., Voskuil, M. I., Gold, B., Schoolnik, G. K., Smith, I. (2002). ideR, an Essential Gene in Mycobacterium tuberculosis: Role of IdeR in Iron-Dependent Gene Expression, Iron Metabolism, and Oxidative Stress Response. Infect. Immun. 70: 3371-3381 [Abstract] [Full Text]  
  • Sassetti, C. M., Boyd, D. H., Rubin, E. J. (2001). Comprehensive identification of conditionally essential genes in mycobacteria. Proc. Natl. Acad. Sci. USA 10.1073/pnas.231275498v1 [Abstract] [Full Text]  
  • Sassetti, C. M., Boyd, D. H., Rubin, E. J. (2001). Comprehensive identification of conditionally essential genes in mycobacteria. Proc. Natl. Acad. Sci. USA 98: 12712-12717 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»