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Journal of Bacteriology, October 2000, p. 5787-5792, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

sigma 54 Promoters Control Expression of Genes Encoding the Hook and Basal Body Complex in Rhodobacter sphaeroides

Sebastian Poggio,1 Carlos Aguilar,1 Aurora Osorio,1 Bertha González-Pedrajo,2 Georges Dreyfus,2 and Laura Camarena1,*

Departamento de Biología Molecular, Instituto de Investigaciones Biomédicas,1 and Departamento de Genética Molecular, Instituto de Fisiología Celular, UNAM,2 04510 Mexico City, Mexico

Received 9 June 2000/Accepted 25 July 2000

Gene expression of the flagellar system is tightly controlled by external stimuli or intracellular signals. A general picture of this regulation has been obtained from studies of Salmonella enterica serovar Typhimurium. However, these regulatory mechanisms do not apply to all bacterial groups. In this study, we have investigated regulation of the flagellar genetic system in Rhodobacter sphaeroides. Deletion analysis, site-directed mutagenesis, and 5'-end mapping were conducted in order to identify the fliO promoter. Our results indicate that this promoter is recognized by the factor sigma 54. Additionally, 5'-end mapping of the flgB and fliK transcripts suggests that these mRNAs are also transcribed from sigma 54 promoters. Finally, we showed evidence that suggests that fliC transcription is not entirely dependent on the presence of a complete basal body-hook structure. Our results are discussed in the context of a possible regulatory hierarchy controlling flagellar gene expression in R. sphaeroides.

* Corresponding author. Mailing address: Departamento de Biología Molecular, Instituto de Investigaciones Biomédicas, UNAM, Ap. Postal 70-228, 04510 Mexico City, Mexico. Phone: (525) 622 38 24. Fax: (525) 622 38 91. E-mail: rosal{at}servidor.unam.mx.

Journal of Bacteriology, October 2000, p. 5787-5792, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pena-Sanchez, J., Poggio, S., Flores-Perez, U., Osorio, A., Domenzain, C., Dreyfus, G., Camarena, L. (2009). Identification of the binding site of the {sigma}54 hetero-oligomeric FleQ/FleT activator in the flagellar promoters of Rhodobacter sphaeroides. Microbiology 155: 1669-1679 [Abstract] [Full Text]  
  • Poggio, S., Osorio, A., Dreyfus, G., Camarena, L. (2006). Transcriptional Specificity of RpoN1 and RpoN2 Involves Differential Recognition of the Promoter Sequences and Specific Interaction with the Cognate Activator Proteins. J. Biol. Chem. 281: 27205-27215 [Abstract] [Full Text]  
  • Ballado, T., Camarena, L., González-Pedrajo, B., Silva-Herzog, E., Dreyfus, G. (2001). The Hook Gene (flgE) Is Expressed from the flgBCDEF Operon in Rhodobacter sphaeroides: Study of an flgE Mutant. J. Bacteriol. 183: 1680-1687 [Abstract] [Full Text]  


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