This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Temmerman, W.
Right arrow Articles by Goethals, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Temmerman, W.
Right arrow Articles by Goethals, K.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2000, p. 5832-5840, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Leafy Gall Formation Is Controlled by fasR, an AraC-Type Regulatory Gene in Rhodococcus fascians

Wim Temmerman, Danny Vereecke, Rozemarijn Dreesen, Marc Van Montagu, Marcelle Holsters,* and Koen Goethals

Vakgroep Moleculaire Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, B-9000 Ghent, Belgium

Received 8 March 2000/Accepted 19 June 2000

Rhodococcus fascians can interact with many plant species and induce the formation of either leafy galls or fasciations. To provoke symptoms, R. fascians strain D188 requires pathogenicity genes that are located on a linear plasmid, pFiD188. The fas genes are essential for virulence and constitute an operon that encodes, among other functions, a cytokinin synthase gene. Expression of the fas genes is induced by extracts of infected plant tissue only. We have isolated an AraC-type regulatory gene, fasR, located on pFiD188, which is indispensable for pathogenesis and for fas gene expression. The combined results of our experiments show that in vitro expression of the fas genes in a defined medium is strictly regulated and that several environmental factors (pH, carbon and nitrogen sources, phosphate and oxygen content, and cell density) and regulatory proteins are involved. We further show that expression of the fas genes is controlled at both the transcriptional and the translational levels. The complex expression pattern probably reflects the necessity of integrating a multitude of signals and underlines the importance of the fas operon in the pathogenicity of R. fascians.


* Corresponding author. Mailing address: Vakgroep Moleculaire Genetica, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium. Phone: (32-9) 264 5170. Fax: (32-9) 264 5349. E-mail: mahol{at}gengenp.rug.ac.be.


Journal of Bacteriology, October 2000, p. 5832-5840, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Depuydt, S., De Veylder, L., Holsters, M., Vereecke, D. (2009). Eternal Youth, the Fate of Developing Arabidopsis Leaves upon Rhodococcus fascians Infection. Plant Physiol. 149: 1387-1398 [Abstract] [Full Text]  
  • Depuydt, S., Trenkamp, S., Fernie, A. R., Elftieh, S., Renou, J.-P., Vuylsteke, M., Holsters, M., Vereecke, D. (2009). An Integrated Genomics Approach to Define Niche Establishment by Rhodococcus fascians. Plant Physiol. 149: 1366-1386 [Abstract] [Full Text]  
  • Pertry, I., Vaclavikova, K., Depuydt, S., Galuszka, P., Spichal, L., Temmerman, W., Stes, E., Schmulling, T., Kakimoto, T., Van Montagu, M. C. E., Strnad, M., Holsters, M., Tarkowski, P., Vereecke, D. (2009). Identification of Rhodococcus fascians cytokinins and their modus operandi to reshape the plant. Proc. Natl. Acad. Sci. USA 106: 929-934 [Abstract] [Full Text]  
  • Kuscer, E., Coates, N., Challis, I., Gregory, M., Wilkinson, B., Sheridan, R., Petkovic, H. (2007). Roles of rapH and rapG in Positive Regulation of Rapamycin Biosynthesis in Streptomyces hygroscopicus. J. Bacteriol. 189: 4756-4763 [Abstract] [Full Text]  
  • Vandeputte, O., Oden, S., Mol, A., Vereecke, D., Goethals, K., El Jaziri, M., Prinsen, E. (2005). Biosynthesis of Auxin by the Gram-Positive Phytopathogen Rhodococcus fascians Is Controlled by Compounds Specific to Infected Plant Tissues. Appl. Environ. Microbiol. 71: 1169-1177 [Abstract] [Full Text]  
  • Brown, S. E., Knudson, D. L., Ishimaru, C. A. (2002). Linear Plasmid in the Genome of Clavibacter michiganensis subsp. sepedonicus. J. Bacteriol. 184: 2841-2844 [Abstract] [Full Text]  
  • Vereecke, D., Cornelis, K., Temmerman, W., Jaziri, M., Van Montagu, M., Holsters, M., Goethals, K. (2002). Chromosomal Locus That Affects Pathogenicity of Rhodococcus fascians. J. Bacteriol. 184: 1112-1120 [Abstract] [Full Text]