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Journal of Bacteriology, October 2000, p. 5880-5884, Vol. 182, No. 20
Department of Genetics and Microbiology,
Facultad de Biologia,1 and Facultad
de Medicina,2 University of Murcia, 30071 Murcia, Spain
Received 12 May 2000/Accepted 26 July 2000
We have characterized an open reading frame of 2,454 bp on
chromosome I of Schizosaccharomyces pombe as the gene
encoding trehalose-6P phosphatase (tpp1+).
Disruption of tpp1+ caused in vivo accumulation
of trehalose-6P upon heat shock and prevented cell growth at 37 to
40°C. Accumulation of trehalose-6P in cells bearing a chromosomal
disruption of the tpp1+ gene and containing a
plasmid with tpp1+ under the control of the
thiamine-repressible promotor correlated with
tpp1+ repression. The level of
tpp1+ mRNA rose upon heat shock,
osmostress, or oxidative stress and was negatively controlled by cyclic
AMP-dependent protein kinase activity. Expression of
tpp1+ during oxidative or osmotic stress, but
not during heat shock, was under positive control by the
wis1-sty1 (equivalent to phh1 and
spc1) mitogen-activated protein kinase pathway. Analysis of Tpp1 protein levels suggests that the synthesis of trehalose-6P phosphatase may also be subjected to translational or posttranslational control.
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Characterization of tpp1+ as Encoding a
Main Trehalose-6P Phosphatase in the Fission Yeast
Schizosaccharomyces pombe
*
Corresponding author. Mailing address: Department of
Genetics and Microbiology, University of Murcia, 30071 Murcia, Spain. Phone: (34) 968 367132. Fax: (34) 968 363963. E-mail:
maga{at}fcu.um.es.
Journal of Bacteriology, October 2000, p. 5880-5884, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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