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Journal of Bacteriology, October 2000, p. 5893-5897, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

SarA Represses agr Operon Expression in a Purified In Vitro Staphylococcus aureus Transcription System

Swarup K. Chakrabarti and Tapan K. Misra*

Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago, Illinois 60612-7344

Received 13 April 2000/Accepted 24 July 2000

Mutation and genetic complementation studies suggested that two chromosomal loci, agr and sar, are involved in the upregulation of several exotoxin genes and the downregulation of a number of surface protein genes in a growth phase-dependent manner in Staphylococcus aureus. We purified recombinant T7-tagged SarA from Escherichia coli and determined its effect on transcription from several S. aureus promoters by using purified RNA polymerase reconstituted with either sigma A or sigma B from S. aureus. Of the seven sigma A-dependent promoters that we tested, SarA repressed transcription from agrP2, agrP3, cna, sarP1, and sea promoters and did not affect sec and znt promoters. Furthermore, SarA had no effect on transcription from the sigma B-dependent sarP3 promoter. In vitro experimental data presented in this report suggest that SarA expression is autoregulated.

* Corresponding author. Mailing address: Department of Microbiology and Immunology (M/C 790), University of Illinois College of Medicine, 835 South Wolcott Ave., Chicago, IL 60612-7344. Phone: (312) 996-9609. Fax: (312) 996-6415. E-mail: tmisra{at}uic.edu.

Journal of Bacteriology, October 2000, p. 5893-5897, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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