Journal of Bacteriology, October 2000, p. 5893-5897, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago, Illinois 60612-7344
Received 13 April 2000/Accepted 24 July 2000
Mutation and genetic complementation studies suggested that two
chromosomal loci, agr and sar, are involved in
the upregulation of several exotoxin genes and the downregulation of a
number of surface protein genes in a growth phase-dependent manner in
Staphylococcus aureus. We purified recombinant T7-tagged
SarA from Escherichia coli and determined its effect on
transcription from several S. aureus promoters by using
purified RNA polymerase reconstituted with either
A or
B from S. aureus. Of the seven
A-dependent promoters that we tested, SarA repressed
transcription from agrP2, agrP3,
cna, sarP1, and sea promoters and
did not affect sec and znt promoters.
Furthermore, SarA had no effect on transcription from the
B-dependent sarP3 promoter. In vitro
experimental data presented in this report suggest that SarA expression
is autoregulated.
This article has been cited by other articles:
| Appl. Environ. Microbiol. | Infect. Immun. | Eukaryot. Cell |
|---|---|---|
| Mol. Cell. Biol. | J. Virol. | Microbiol. Mol. Biol. Rev. |
| ALL ASM JOURNALS |