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Journal of Bacteriology, October 2000, p. 5911-5915, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Organization and Expression of a Thermus thermophilus Arginine Cluster: Presence of Unidentified Open Reading Frames and Absence of a Shine-Dalgarno Sequence

Rony Sanchez,1 Martine Roovers,1 and Nicolas Glansdorff1,2,3,*

Department of Microbiology, Flanders Interuniversity Institute for Biotechnology (VIB),1 Department of Microbiology, Free University of Brussels (VUB),2 and Research Institute J. M. Wiame,3 1070 Brussels, Belgium

Received 18 April 2000/Accepted 26 July 2000

A group of genes regulated by arginine was found clustered in the order argF-ORF1-argC-argJ-ORF4 between other, as yet uncharacterized, open reading frames (ORFs). Transcription starts were identified immediately upstream from argF and ORF4. Arginine repressed transcription that was initiated at argF but induced transcription of ORF4. The functions of ORF1 and ORF4 are unknown, but analysis of the sequence of ORF4 suggests that it is a membrane protein, possibly involved in transport of arginine or a related metabolite. Mobility shift and DNase I footprinting have revealed specific binding of pure Escherichia coli ArgR to the promoter region of Thermus thermophilus argF. These results suggest that argF transcription is controlled by a repressor homologous to those characterized in enteric bacteria and bacilli. Thermus argF mRNA is devoid of Shine-Dalgarno (SD) sequences. However, downstream from the ATG start codon of argF and many other Thermus genes (with or without an SD box), sequences were found to be complementary to nucleotides 1392 to 1409 of Thermus 16S rRNA, suggesting that an mRNA-rRNA base pairing in this region is important for correct translation initiation.

* Corresponding author. Mailing address: Flanders Interuniversity Institute for Biotechnology (VIB), Free University of Brussels (VUB), and Research Institute J. M. Wiame, E. Grysonlaan 1, 1070 Brussels, Belgium. Phone: 32 2 5267275. Fax: 32 2 5267273. E-mail: ceriair{at}ulb.ac.be.

Journal of Bacteriology, October 2000, p. 5911-5915, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Fujiwara, K., Tsubouchi, T., Kuzuyama, T., Nishiyama, M. (2006). Involvement of the arginine repressor in lysine biosynthesis of Thermus thermophilus. Microbiology 152: 3585-3594 [Abstract] [Full Text]  
  • Moll, I., Huber, M., Grill, S., Sairafi, P., Mueller, F., Brimacombe, R., Londei, P., Bläsi, U. (2001). Evidence against an Interaction between the mRNA Downstream Box and 16S rRNA in Translation Initiation. J. Bacteriol. 183: 3499-3505 [Abstract] [Full Text]  


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