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Journal of Bacteriology, October 2000, p. 5916-5918, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Mobilization of Poly(3-Hydroxybutyrate) in
Ralstonia eutropha
René
Handrick,
Simone
Reinhardt, and
Dieter
Jendrossek*
Institut für Mikrobiologie,
Universität Stuttgart, 70550 Stuttgart, Germany
Received 17 April 2000/Accepted 19 July 2000
Ralstonia eutropha H16 degraded (mobilized) previously
accumulated poly(3-hydroxybutyrate) (PHB) in the absence of an
exogenous carbon source and used the degradation products for growth
and survival. Isolated native PHB granules of mobilized R. eutropha cells released 3-hydroxybutyrate (3HB) at a threefold
higher rate than did control granules of nonmobilized bacteria. No 3HB
was released by native PHB granules of recombinant Escherichia
coli expressing the PHB biosynthetic genes. Native PHB granules
isolated from chromosomal knockout mutants of an intracellular PHB
(i-PHB) depolymerase gene of R. eutropha H16 and HF210
showed a reduced but not completely eliminated activity of 3HB release
and indicated the presence of i-PHB depolymerase isoenzymes.
*
Corresponding author. Mailing address: Institut
für Mikrobiologie, Universität Stuttgart, Allmandring 31, 70550 Stuttgart, Germany. Phone: 49-711-685-5483. Fax:
49-711-685-5725. E-mail: dieter.jendrossek{at}po.uni-stuttgart.de.
Journal of Bacteriology, October 2000, p. 5916-5918, Vol. 182, No. 20
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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