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Journal of Bacteriology, November 2000, p. 5948-5953, Vol. 182, No. 21
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Regulation of Ferritin-Mediated Cytoplasmic Iron Storage by the
Ferric Uptake Regulator Homolog (Fur) of Helicobacter
pylori
Stefan
Bereswill,1,*
Stefan
Greiner,1
Arnoud H. M.
van
Vliet,2
Barbara
Waidner,1
Frank
Fassbinder,1,3
Emile
Schiltz,4
Johannes G.
Kusters,2 and
Manfred
Kist1
Department of Microbiology and Hygiene,
Institute of Medical Microbiology and Hygiene,1
Institute of Microbiology,3 and
Institute of Organic Chemistry and
Biochemistry,4 University of Freiburg, D-79104
Freiburg, Germany, and Faculty of Medicine, Department of
Medical Microbiology, Vrije Universiteit, 1081 BT Amsterdam, The
Netherlands2
Received 12 April 2000/Accepted 1 August 2000
Homologs of the ferric uptake regulator Fur and the iron storage
protein ferritin play a central role in maintaining iron homeostasis in
bacteria. The gastric pathogen Helicobacter pylori contains
an iron-induced prokaryotic ferritin (Pfr) which has been shown to be
involved in protection against metal toxicity and a Fur homolog which
has not been functionally characterized in H. pylori.
Analysis of an isogenic fur-negative mutant revealed that
H. pylori Fur is required for metal-dependent regulation of
ferritin. Iron starvation, as well as medium supplementation with
nickel, zinc, copper, and manganese at nontoxic concentrations, repressed synthesis of ferritin in the wild-type strain but not in the
H. pylori fur mutant. Fur-mediated regulation of ferritin synthesis occurs at the mRNA level. With respect to the regulation of
ferritin expression, Fur behaves like a global metal-dependent repressor which is activated under iron-restricted conditions but also
responds to different metals. Downregulation of ferritin expression by
Fur might secure the availability of free iron in the cytoplasm,
especially if iron is scarce or titrated out by other metals.
*
Corresponding author. Mailing address: University of
Freiburg, Institute of Medical Microbiology and Hygiene, Department of Microbiology, Hermann-Herder-Str. 11, D-79104 Freiburg, Germany. Phone:
49-761-203-6539. Fax: 49-761-203-6562. E-mail:
bereswil{at}ukl.uni-freiburg.de.
Journal of Bacteriology, November 2000, p. 5948-5953, Vol. 182, No. 21
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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