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Journal of Bacteriology, November 2000, p. 6123-6129, Vol. 182, No. 21
Institut für Mikrobiologie,
Universität Stuttgart, 70569 Stuttgart, Germany
Received 24 April 2000/Accepted 4 July 2000
The genes for two different protocatechuate 3,4-dioxygenases
(P34Os) were cloned from the 4-sulfocatechol-degrading bacterium Agrobacterium radiobacter strain S2 (DSMZ 5681). The
pcaH1G1 genes encoded a P34O (P34O-I) which oxidized
protocatechuate but not 4-sulfocatechol. These genes were part of a
protocatechuate-degradative operon which strongly resembled the
isofunctional operon from the protocatechuate-degrading strain
Agrobacterium tumefaciens A348 described previously by D. Parke (FEMS Microbiol. Lett. 146:3-12, 1997). The second P34O
(P34O-II), encoded by the pcaH2G2 genes, was functionally
expressed and shown to convert protocatechuate and 4-sulfocatechol. A
comparison of the deduced amino acid sequences of PcaH-I and PcaH-II,
and of PcaG-I and PcaG-II, with each other and with the corresponding
sequences from the P34Os, from other bacterial genera suggested that
the genes for the P34O-II were obtained by strain S2 by lateral gene
transfer. The genes encoding the P34O-II were found in a putative
operon together with two genes which, according to sequence alignments,
encoded transport proteins. Further downstream from this putative
operon, two open reading frames which code for a putative regulator
protein of the IclR family and a putative 3-carboxymuconate
cycloisomerase were identified.
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Characterization of the Genes for Two
Protocatechuate 3,4-Dioxygenases from the
4-Sulfocatechol-Degrading Bacterium Agrobacterium
radiobacter Strain S2
*
Corresponding author. Mailing address: Institut
für Mikrobiologie, Universität Stuttgart, 70569 Stuttgart,
Germany. Phone: 49-711-6855489. Fax: 49-711-6855725. E-mail:
Andreas.Stolz{at}PO.Uni-Stuttgart.DE.
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