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Journal of Bacteriology, November 2000, p. 6222-6227, Vol. 182, No. 21
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Mapping of the Cryptococcus neoformans MATalpha Locus: Presence of Mating Type-Specific Mitogen-Activated Protein Kinase Cascade Homologs

M. Karos,1 Y. C. Chang,1 C. M. McClelland,2 D. L. Clarke,2 J. Fu,2 B. L. Wickes,2 and K. J. Kwon-Chung1,*

Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892,1 and Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229-39002

Received 16 June 2000/Accepted 16 August 2000

In this study we investigated the relationship between the MATalpha locus of Cryptococcus neoformans and several MATalpha -specific mitogen-activated protein (MAP) kinase signal transduction cascade genes, including STE12alpha , STE11alpha , and STE20alpha . To resolve the location of the genes, we screened a cosmid library of the MATalpha strain B-4500 (JEC21), which was chosen for the C. neoformans genome project. We isolated several overlapping cosmids spanning a region of about 71 kb covering the entire MATalpha locus. It was found that STE12alpha , STE11alpha , and STE20alpha are imbedded within the locus rather than closely linked to the locus. Furthermore, three copies of MFalpha , the mating type alpha -pheromone gene, a MATalpha -specific myosin gene, and a pheromone receptor (CPRalpha ) were identified within the locus. We created a physical map, based on the restriction enzyme BamHI, and identified both borders of the MATalpha locus. The MATalpha locus of C. neoformans is approximately 50 kb in size and is one of the largest mating type loci reported among fungi with a one-locus, two-allele mating system.

* Corresponding author. Mailing address: Building 10, Room 11C304, National Institutes of Health, Bethesda, MD 20892. Phone: (301) 496-1602. Fax: (301) 402-1003. E-mail: June_Kwon-Chung{at}NIH.GOV.

Journal of Bacteriology, November 2000, p. 6222-6227, Vol. 182, No. 21
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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