This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Karos, M.
Right arrow Articles by Kwon-Chung, K. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Karos, M.
Right arrow Articles by Kwon-Chung, K. J.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 2000, p. 6222-6227, Vol. 182, No. 21
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Mapping of the Cryptococcus neoformans MATalpha Locus: Presence of Mating Type-Specific Mitogen-Activated Protein Kinase Cascade Homologs

M. Karos,1 Y. C. Chang,1 C. M. McClelland,2 D. L. Clarke,2 J. Fu,2 B. L. Wickes,2 and K. J. Kwon-Chung1,*

Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892,1 and Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229-39002

Received 16 June 2000/Accepted 16 August 2000

In this study we investigated the relationship between the MATalpha locus of Cryptococcus neoformans and several MATalpha -specific mitogen-activated protein (MAP) kinase signal transduction cascade genes, including STE12alpha , STE11alpha , and STE20alpha . To resolve the location of the genes, we screened a cosmid library of the MATalpha strain B-4500 (JEC21), which was chosen for the C. neoformans genome project. We isolated several overlapping cosmids spanning a region of about 71 kb covering the entire MATalpha locus. It was found that STE12alpha , STE11alpha , and STE20alpha are imbedded within the locus rather than closely linked to the locus. Furthermore, three copies of MFalpha , the mating type alpha -pheromone gene, a MATalpha -specific myosin gene, and a pheromone receptor (CPRalpha ) were identified within the locus. We created a physical map, based on the restriction enzyme BamHI, and identified both borders of the MATalpha locus. The MATalpha locus of C. neoformans is approximately 50 kb in size and is one of the largest mating type loci reported among fungi with a one-locus, two-allele mating system.

* Corresponding author. Mailing address: Building 10, Room 11C304, National Institutes of Health, Bethesda, MD 20892. Phone: (301) 496-1602. Fax: (301) 402-1003. E-mail: June_Kwon-Chung{at}NIH.GOV.

Journal of Bacteriology, November 2000, p. 6222-6227, Vol. 182, No. 21
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ren, P., Springer, D. J., Behr, M. J., Samsonoff, W. A., Chaturvedi, S., Chaturvedi, V. (2006). Transcription Factor STE12{alpha} Has Distinct Roles in Morphogenesis, Virulence, and Ecological Fitness of the Primary Pathogenic Yeast Cryptococcus gattii.. Eukaryot Cell 5: 1065-1080 [Abstract] [Full Text]  
  • Weber, I., Gruber, C., Steinberg, G. (2003). A Class-V Myosin Required for Mating, Hyphal Growth, and Pathogenicity in the Dimorphic Plant Pathogen Ustilago maydis. Plant Cell 15: 2826-2842 [Abstract] [Full Text]  
  • Chang, Y. C., Miller, G. F., Kwon-Chung, K. J. (2003). Importance of a Developmentally Regulated Pheromone Receptor of Cryptococcus neoformans for Virulence. Infect. Immun. 71: 4953-4960 [Abstract] [Full Text]  
  • Hull, C. M., Davidson, R. C., Heitman, J. (2002). Cell identity and sexual development in Cryptococcus neoformans are controlled by the mating-type-specific homeodomain protein Sxi1alpha. Genes Dev. 16: 3046-3060 [Abstract] [Full Text]  
  • Mylonakis, E., Ausubel, F. M., Perfect, J. R., Heitman, J., Calderwood, S. B. (2002). Killing of Caenorhabditiselegans by Cryptococcus neoformans as a model of yeast pathogenesis. Proc. Natl. Acad. Sci. USA 99: 15675-15680 [Abstract] [Full Text]  
  • Lengeler, K. B., Fox, D. S., Fraser, J. A., Allen, A., Forrester, K., Dietrich, F. S., Heitman, J. (2002). Mating-Type Locus of Cryptococcus neoformans: a Step in the Evolution of Sex Chromosomes. Eukaryot Cell 1: 704-718 [Abstract] [Full Text]  
  • Chaturvedi, V., Fan, J., Stein, B., Behr, M. J., Samsonoff, W. A., Wickes, B. L., Chaturvedi, S. (2002). Molecular Genetic Analyses of Mating Pheromones Reveal Intervariety Mating or Hybridization in Cryptococcus neoformans. Infect. Immun. 70: 5225-5235 [Abstract] [Full Text]  
  • Schein, J. E., Tangen, K. L., Chiu, R., Shin, H., Lengeler, K. B., MacDonald, W. K., Bosdet, I., Heitman, J., Jones, S. J.M., Marra, M. A., Kronstad, J. W. (2002). Physical Maps for Genome Analysis of Serotype A and D Strains of the Fungal Pathogen Cryptococcus neoformans. Genome Res 12: 1445-1453 [Abstract] [Full Text]  
  • Shen, W.-C., Davidson, R. C., Cox, G. M., Heitman, J. (2002). Pheromones Stimulate Mating and Differentiation via Paracrine and Autocrine Signaling in Cryptococcus neoformans. Eukaryot Cell 1: 366-377 [Abstract] [Full Text]  
  • Chung, S., Karos, M., Chang, Y. C., Lukszo, J., Wickes, B. L., Kwon-Chung, K. J. (2002). Molecular Analysis of CPR{alpha}, a MAT{alpha}-Specific Pheromone Receptor Gene of Cryptococcus neoformans. Eukaryot Cell 1: 432-439 [Abstract] [Full Text]  
  • McClelland, C. M., Fu, J., Woodlee, G. L., Seymour, T. S., Wickes, B. L. (2002). Isolation and Characterization of the Cryptococcus neoformans MATa Pheromone Gene. Genetics 160: 935-947 [Abstract] [Full Text]  
  • Cogliati, M., Esposto, M. C., Clarke, D. L., Wickes, B. L., Viviani, M. A. (2001). Origin of Cryptococcus neoformans var. neoformans Diploid Strains. J. Clin. Microbiol. 39: 3889-3894 [Abstract] [Full Text]  
  • Smulian, A. G., Sesterhenn, T., Tanaka, R., Cushion, M. T. (2001). The ste3 Pheromone Receptor Gene of Pneumocystis carinii Is Surrounded by a Cluster of Signal Transduction Genes. Genetics 157: 991-1002 [Abstract] [Full Text]  
  • Chang, Y. C., Penoyer, L. A., Kwon-Chung, K. J. (2001). The second STE12 homologue of Cryptococcus neoformans is MATa-specific and plays an important role in virulence. Proc. Natl. Acad. Sci. USA 10.1073/pnas.061031998v1 [Abstract] [Full Text]  
  • Chang, Y. C., Penoyer, L. A., Kwon-Chung, K. J. (2001). The second STE12 homologue of Cryptococcus neoformans is MATa-specific and plays an important role in virulence. Proc. Natl. Acad. Sci. USA 98: 3258-3263 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»