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Journal of Bacteriology, November 2000, p. 6374-6381, Vol. 182, No. 22
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Complementary Metal Ion Specificity of the
Metal-Citrate Transporters CitM and CitH of Bacillus
subtilis
Bastiaan P.
Krom,
Jessica B.
Warner,
Wil N.
Konings, and
Juke S.
Lolkema*
Department of Microbiology, Groningen
Biomolecular Sciences and Biotechnology Institute, University of
Groningen, Haren, The Netherlands
Received 28 June 2000/Accepted 23 August 2000
Citrate uptake in Bacillus subtilis is stimulated by a
wide range of divalent metal ions. The metal ions were separated into two groups based on the expression pattern of the uptake system. The
two groups correlated with the metal ion specificity of two homologous
B. subtilis secondary citrate transporters, CitM and CitH,
upon expression in Escherichia coli. CitM transported
citrate in complex with Mg2+, Ni2+,
Mn2+, Co2+, and Zn2+ but not in
complex with Ca2+, Ba2+, and Sr2+.
CitH transported citrate in complex with Ca2+,
Ba2+, and Sr2+ but not in complex with
Mg2+, Ni2+, Mn2+, Co2+,
and Zn2+. Both transporters did not transport free citrate.
Nevertheless, free citrate uptake could be demonstrated in B. subtilis, indicating the expression of at least a third citrate
transporter, whose identity is not known. For both the CitM and CitH
transporters it was demonstrated that the metal ion promoted citrate
uptake and, vice versa, that citrate promoted uptake of the metal ion, indicating that the complex is the transported species. The results indicate that CitM and CitH are secondary transporters that transport complexes of divalent metal ions and citrate but with a complementary metal ion specificity. The potential physiological function of the two
transporters is discussed.
*
Corresponding author. Mailing address: Department of
Microbiology, University of Groningen, Biological Center, Kerklaan 30, 9751 NN Haren, The Netherlands. Phone: (31)-50-3632150. Fax:
(31)-50-3632154. E-mail: j.s.lolkema{at}biol.rug.nl.
Journal of Bacteriology, November 2000, p. 6374-6381, Vol. 182, No. 22
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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