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Journal of Bacteriology, November 2000, p. 6401-6411, Vol. 182, No. 22
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
The Pseudomonas aeruginosa Lectins PA-IL
and PA-IIL Are Controlled by Quorum Sensing and by RpoS
Klaus
Winzer,1,2
Colin
Falconer,1
Nachman C.
Garber,3
Stephen P.
Diggle,1
Miguel
Camara,1 and
Paul
Williams1,2,*
School of Pharmaceutical
Sciences1 and Institute of Infections
and Immunity,2 University of Nottingham,
University Park, Nottingham NG7 2RD, United Kingdom, and
Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52 900, Israel3
Received 15 May 2000/Accepted 5 September 2000
In Pseudomonas aeruginosa, many exoproduct virulence
determinants are regulated via a hierarchical quorum-sensing cascade involving the transcriptional regulators LasR and RhlR and their cognate activators,
N-(3-oxododecanoyl)-L-homoserine lactone
(3O-C12-HSL) and N-butanoyl-L-homoserine
lactone (C4-HSL). In this paper, we demonstrate that the cytotoxic
lectins PA-IL and PA-IIL are regulated via quorum sensing. Using
immunoblot analysis, the production of both lectins was found to be
directly dependent on the rhl locus while, in a
lasR mutant, the onset of lectin synthesis was delayed but
not abolished. The PA-IL structural gene, lecA, was cloned
and sequenced. Transcript analysis indicated a monocistronic organization with a transcriptional start site 70 bp upstream of the
lecA translational start codon. A lux box-type
element together with RpoS (
S) consensus sequences was
identified upstream of the putative promoter region. In
Escherichia coli, expression of a
lecA::lux reporter fusion was
activated by RhlR/C4-HSL, but not by LasR/3O-C12-HSL, confirming direct
regulation by RhlR/C4-HSL. Similarly, in P. aeruginosa
PAO1, the expression of a chromosomal
lecA::lux fusion was enhanced but not
advanced by the addition of exogenous C4-HSL but not 3O-C12-HSL.
Furthermore, mutation of rpoS abolished lectin synthesis in
P. aeruginosa, demonstrating that both RpoS and
RhlR/C4-HSL are required. Although the C4-HSL-dependent expression
of the lecA::lux reporter in E. coli could be inhibited by the presence of 3O-C12-HSL, this did
not occur in P. aeruginosa. This suggests that, in the
homologous genetic background, 3O-C12-HSL does not function as a
posttranslational regulator of the RhlR/C4-HSL-dependent activation of
lecA expression.
*
Corresponding author. Mailing address: School of
Pharmaceutical Sciences, University Park, University of
Nottingham, Nottingham NG7 2RD, United Kingdom. Phone: (44)115
9515047. Fax: (44)115 8466296. E-mail:
paul.williams{at}nottingham.ac.uk.
Journal of Bacteriology, November 2000, p. 6401-6411, Vol. 182, No. 22
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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