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Journal of Bacteriology, November 2000, p. 6451-6455, Vol. 182, No. 22
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Novel Toluene Elimination System in a Toluene-Tolerant Microorganism

Hideki Kobayashi,1,* Katsuyuki Uematsu,2 Hisako Hirayama,1 and Koki Horikoshi1,3

Japan Marine Science and Technology Center, Deep-Sea Microorganisms Research Group, Yokosuka 237-0061,1 Marine Works Japan Ltd., Technical Support Division, Live Pier Kanazawahakkei 1-1-7 Mutsuura, Kanazawa-ku, Yokohama 236-0031,2 and Department of Applied Chemistry, Faculty of Engineering, Toyo University, 2100 Kujirai, Kawagoe, Saitama 350-8585,3 Japan

Received 19 April 2000/Accepted 30 August 2000

In studies of Pseudomonas putida IH-2000, a toluene-tolerant microorganism, membrane vesicles (MVs) were found to be released from the outer membrane when toluene was added to the culture. These MVs were found to be composed of phospholipids, lipopolysaccharides (LPS), and very low amounts of outer membrane proteins. The MVs also contained a higher concentration of toluene molecules (0.172 ± 0.012 mol/mol of lipid) than that found in the cell membrane. In contrast to the wild-type strain, the toluene-sensitive mutant strain 32, which differs from the parent strain in LPS and outer membrane proteins, did not release MVs from the outer membrane. The toluene molecules adhering to the outer membrane are eliminated by the shedding of MVs, and this system appears to serve as an important part of the toluene tolerance system of IH-2000.

* Corresponding author. Mailing address: Japan Marine Science and Technology Center, Deep-Sea Microorganisms Research Group, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan. Phone: 81-468-67-5556. Fax: 81-468-66-6364. E-mail: hidekik{at}jamstec.go.jp.

Journal of Bacteriology, November 2000, p. 6451-6455, Vol. 182, No. 22
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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