This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kurdi, P. Articles by Yokota, A. Search for Related Content PubMed PubMed Citation Articles by Kurdi, P. Articles by Yokota, A.

Journal of Bacteriology, November 2000, p. 6525-6528, Vol. 182, No. 22
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cholic Acid Is Accumulated Spontaneously, Driven by Membrane pH, in Many Lactobacilli

Peter Kurdi,¹ Hendrik W. van Veen,² Hiroshi Tanaka,³ Igor Mierau,^3, Wil N. Konings,² Gerald W. Tannock,⁴ Fusao Tomita,¹ and Atsushi Yokota^5,*

Laboratory of Applied Microbiology¹ and Laboratory of Microbial Resources and Ecology,⁵ Research Group of Molecular Bioscience, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan; Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren,² and Snow Brand European Research Laboratories B.V., 9747 AN Groningen,³ The Netherlands; and Department of Microbiology, University of Otago, Dunedin, New Zealand⁴

Received 1 May 2000/Accepted 30 August 2000

Many lactobacilli from various origins were found to apparently lack cholic acid extrusion activity. Cholic acid was accumulated spontaneously, driven by the transmembrane proton gradient. Accumulation is a newly identified kind of interaction between intestinal microbes and unconjugated bile acids and is different from extrusion and modification, which have been described previously.

---

^* Corresponding author. Mailing address: Laboratory of Microbial Resources and Ecology, Graduate School of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kita-ku, Sapporo 060-8589, Japan. Phone: 81-11-706-2501. Fax: 81-11-706-4961. E-mail: yokota{at}chem.agr.hokudai.ac.jp.

Present address: NIZO Food Research, Ede, The Netherlands.

---

Journal of Bacteriology, November 2000, p. 6525-6528, Vol. 182, No. 22
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

• Begley, M., Hill, C., Gahan, C. G. M. (2006). Bile salt hydrolase activity in probiotics.. Appl. Environ. Microbiol. 72: 1729-1738 [Full Text]  
• Kurdi, P., Kawanishi, K., Mizutani, K., Yokota, A. (2006). Mechanism of growth inhibition by free bile acids in lactobacilli and bifidobacteria.. J. Bacteriol. 188: 1979-1986 [Abstract] [Full Text]  
• Ridlon, J. M., Kang, D.-J., Hylemon, P. B. (2006). Bile salt biotransformations by human intestinal bacteria. J. Lipid Res. 47: 241-259 [Abstract] [Full Text]  
• Liong, M. T., Shah, N. P. (2005). Acid and Bile Tolerance and Cholesterol Removal Ability of Lactobacilli Strains. J DAIRY SCI 88: 55-66 [Abstract] [Full Text]  
• Elkins, C. A., Mullis, L. B. (2004). Bile-Mediated Aminoglycoside Sensitivity in Lactobacillus Species Likely Results from Increased Membrane Permeability Attributable to Cholic Acid. Appl. Environ. Microbiol. 70: 7200-7209 [Abstract] [Full Text]  
• Kurdi, P., Tanaka, H., van Veen, H. W., Asano, K., Tomita, F., Yokota, A. (2003). Cholic acid accumulation and its diminution by short-chain fatty acids in bifidobacteria. Microbiology 149: 2031-2037 [Abstract] [Full Text]  
• Dean, M., Cervellati, C., Casanova, E., Squerzanti, M., Lanzara, V., Medici, A., Polverino de Laureto, P., Bergamini, C. M. (2002). Characterization of Cholylglycine Hydrolase from a Bile-Adapted Strain of Xanthomonas maltophilia and Its Application for Quantitative Hydrolysis of Conjugated Bile Salts. Appl. Environ. Microbiol. 68: 3126-3128 [Abstract] [Full Text]