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Journal of Bacteriology, November 2000, p. 6532-6535, Vol. 182, No. 22
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Analysis of the Role of recA in Phenotypic Switching of Pseudomonas tolaasii

Himanshu Sinha,dagger Arnab Pain,Dagger and Keith Johnstone*

Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom

Received 22 May 2000/Accepted 25 August 2000

Switching between the pathogenic smooth (1116S) and nonpathogenic rough (1116R) forms of Pseudomonas tolaasii occurs due to the reversible duplication of a 661-bp element within the pheN locus. Disruption of the chromosomal recA locus of 1116S and 1116R produced strains 1116SrecA and 1116RrecA, respectively, which showed typical loss of UV resistance. Switching from the smooth to the rough form was virtually eliminated in the 1116SrecA strain, whereas the extent of switching from the rough to the smooth form was almost identical in 1116R and 1116RrecA. It is concluded that phenotypic switching from 1116S to 1116R is recA dependent whereas that from 1116R to 1116S is recA independent.

* Corresponding author. Mailing address: Department of Plant Sciences, University of Cambridge, Downing St., Cambridge CB2 3EA, United Kingdom. Phone: 44 1223 333933. Fax: 44 1223 333953. E-mail: kj10{at}cam.ac.uk.

dagger Present address: Department of Microbiology, Duke University Medical Center, Durham NC 27710.

Dagger Present address: Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DS, United Kingdom.

Journal of Bacteriology, November 2000, p. 6532-6535, Vol. 182, No. 22
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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