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Journal of Bacteriology, November 2000, p. 6536-6540, Vol. 182, No. 22
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of an Ancillary Protein, YabF, Required for Activity of the KefC Glutathione-Gated Potassium Efflux System in Escherichia coli

S. Miller, L. S. Ness, C. M. Wood, B. C. Fox, and I. R. Booth*

Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, United Kingdom

Received 27 June 2000/Accepted 28 August 2000

A new subunit, YabF, for the KefC K+ efflux system in Escherichia coli has been identified. The subunit is required for maximum activity of KefC. Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations.

* Corresponding author. Mailing address: Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, United Kingdom. Phone: 44-1224-273152. Fax: 44-1224-273144. E-mail: gen118{at}abdn.ac.uk.

Journal of Bacteriology, November 2000, p. 6536-6540, Vol. 182, No. 22
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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