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Journal of Bacteriology, December 2000, p. 6598-6604, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Escherichia coli Responses to a Single DNA Adduct

Gagan A. Pandya,dagger In-Young Yang, Arthur P. Grollman, and Masaaki Moriya*

Laboratory of Chemical Biology, Department of Pharmacological Sciences, SUNY at Stony Brook, Stony Brook, New York 11794-8651

Received 14 June 2000/Accepted 12 September 2000

To study the mechanisms by which Escherichia coli modulates the genotoxic effects of DNA damage, a novel system has been developed which permits quantitative measurements of various E. coli pathways involved in mutagenesis and DNA repair. Events measured include fidelity and efficiency of translesion DNA synthesis, excision repair, and recombination repair. Our strategy involves heteroduplex plasmid DNA bearing a single site-specific DNA adduct and several mismatched regions. The plasmid replicates in a mismatch repair-deficient host with the mismatches serving as strand-specific markers. Analysis of progeny plasmid DNA for linkage of the strand-specific markers identifies the pathway from which the plasmid is derived. Using this approach, a single 1,N6-ethenodeoxyadenosine adduct was shown to be repaired inefficiently by excision repair, to inhibit DNA synthesis by approximately 80 to 90%, and to direct the incorporation of correct dTMP opposite this adduct. This approach is especially useful in analyzing the damage avoidance-tolerance mechanisms. Our results also show that (i) progeny derived from the damage avoidance-tolerance pathway(s) accounts for more than 15% of all progeny; (ii) this pathway(s) requires functional recA, recF, recO, and recR genes, suggesting the mechanism to be daughter strand gap repair; (iii) the ruvABC genes or the recG gene is also required; and (iv) the RecG pathway appears to be more active than the RuvABC pathway. Based on these results, the mechanism of the damage avoidance-tolerance pathway is discussed.

* Corresponding author. Mailing address: Laboratory of Chemical Biology, Department of Pharmacological Sciences, SUNY at Stony Brook, Stony Brook, NY 11794-8651. Phone: (631) 444-3082. Fax: (631) 444-7641. E-mail: maki{at}pharm.sunysb.edu.

dagger Present address: Wyeth-Ayerst Research, Pearl River, NY 10965.

Journal of Bacteriology, December 2000, p. 6598-6604, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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