This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Garza, A. G.
Right arrow Articles by Singer, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Garza, A. G.
Right arrow Articles by Singer, M.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2000, p. 6622-6629, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Control of asgE Expression during Growth and Development of Myxococcus xanthus

Anthony G. Garza,dagger Baruch Z. Harris,Dagger Brandon M. Greenberg,§ and Mitchell Singer*

Section of Microbiology, University of California, Davis, Davis, California 95616

Received 8 May 2000/Accepted 21 September 2000

One of the earliest events in the Myxococcus xanthus developmental cycle is production of an extracellular cell density signal called A-signal (or A-factor). Previously, we showed that cells carrying an insertion in the asgE gene fail to produce normal levels of this cell-cell signal. In this study we found that expression of asgE is growth phase regulated and developmentally regulated. Several lines of evidence indicate that asgE is cotranscribed with an upstream gene during development. Using primer extension analyses, we identified two 5' ends for this developmental transcript. The DNA sequence upstream of one 5' end has similarity to the promoter regions of several genes that are A-signal dependent, whereas sequences located upstream of the second 5' end show similarity to promoter elements identified for genes that are C-signal dependent. Consistent with this result is our finding that mutants failing to produce A-signal or C-signal are defective for developmental expression of asgE. In contrast to developing cells, the large majority of the asgE transcript found in vegetative cells appears to be monocistronic. This finding suggests that asgE uses different promoters for expression during vegetative growth and development. Growth phase regulation of asgE is abolished in a relA mutant, indicating that this vegetative promoter is induced by starvation. The data presented here, in combination with our previous results, indicate that the level of AsgE in vegetative cells is sufficient for this protein to carry out its function during development.

* Corresponding author. Mailing address: Section of Microbiology, One Shields Ave., University of California, Davis, Davis, CA 95616. Phone: (530) 752-9005. Fax: (530) 752-9014. E-mail: mhsinger{at}ucdavis.edu.

dagger Present address: Departments of Biochemistry and Developmental Biology, Stanford University, Stanford, CA 94305.

Dagger Present address: Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143.

§ Department of Microbiology and Molecular Genetics, The University of Texas Medical School, Houston, TX 77225.

Journal of Bacteriology, December 2000, p. 6622-6629, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ossa, F., Diodati, M. E., Caberoy, N. B., Giglio, K. M., Edmonds, M., Singer, M., Garza, A. G. (2007). The Myxococcus xanthus Nla4 Protein Is Important for Expression of Stringent Response-Associated Genes, ppGpp Accumulation, and Fruiting Body Development. J. Bacteriol. 189: 8474-8483 [Abstract] [Full Text]  
  • Viswanathan, P., Singer, M., Kroos, L. (2006). Role of {sigma}D in Regulating Genes and Signals during Myxococcus xanthus Development. J. Bacteriol. 188: 3246-3256 [Abstract] [Full Text]  
  • Diodati, M. E., Ossa, F., Caberoy, N. B., Jose, I. R., Hiraiwa, W., Igo, M. M., Singer, M., Garza, A. G. (2006). Nla18, a Key Regulatory Protein Required for Normal Growth and Development of Myxococcus xanthus.. J. Bacteriol. 188: 1733-1743 [Abstract] [Full Text]  
  • Yoder, D. R., Kroos, L. (2004). Mutational Analysis of the Myxococcus xanthus {Omega}4499 Promoter Region Reveals Shared and Unique Properties in Comparison with Other C-Signal-Dependent Promoters. J. Bacteriol. 186: 3766-3776 [Abstract] [Full Text]  
  • Brenner, M., Garza, A. G., Singer, M. (2004). nsd, a Locus That Affects the Myxococcus xanthus Cellular Response to Nutrient Concentration. J. Bacteriol. 186: 3461-3471 [Abstract] [Full Text]  
  • Jin, W., Ryu, Y. G., Kang, S. G., Kim, S. K., Saito, N., Ochi, K., Lee, S. H., Lee, K. J. (2004). Two relA/spoT homologous genes are involved in the morphological and physiological differentiation of Streptomyces clavuligerus. Microbiology 150: 1485-1493 [Abstract] [Full Text]  
  • Caberoy, N. B., Welch, R. D., Jakobsen, J. S., Slater, S. C., Garza, A. G. (2003). Global Mutational Analysis of NtrC-Like Activators in Myxococcus xanthus: Identifying Activator Mutants Defective for Motility and Fruiting Body Development. J. Bacteriol. 185: 6083-6094 [Abstract] [Full Text]  
  • Mechold, U., Murphy, H., Brown, L., Cashel, M. (2002). Intramolecular Regulation of the Opposing (p)ppGpp Catalytic Activities of RelSeq, the Rel/Spo Enzyme from Streptococcus equisimilis. J. Bacteriol. 184: 2878-2888 [Abstract] [Full Text]  
  • Tse, H., Gill, R. E. (2002). Bypass of A- and B-Signaling Requirements for Myxococcus xanthus Development by Mutations in spdR. J. Bacteriol. 184: 1455-1457 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»