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Journal of Bacteriology, December 2000, p. 6694-6697, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

NorM of Vibrio parahaemolyticus Is an Na+-Driven Multidrug Efflux Pump

Yuji Morita, Atsuko Kataoka, Sumiko Shiota, Tohru Mizushima, and Tomofusa Tsuchiya*

Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, 700-8530, Japan

Received 19 June 2000/Accepted 15 September 2000

NorM of Vibrio parahaemolyticus apparently is a new type of multidrug efflux protein, with no significant sequence similarity to any known transport proteins. Based on the following experimental results, we conclude that NorM is an Na+-driven Na+/drug antiporter. (i) Energy-dependent ethidium efflux from cells possessing NorM was observed in the presence of Na+ but not of K+. (ii) An artificially imposed, inwardly directed Na+ gradient elicited ethidium efflux from cells. (iii) The addition of ethidium to cells loaded with Na+ elicited Na+ efflux. Thus, NorM is an Na+/drug antiporting multidrug efflux pump, the first to be found in the biological world. Judging from the similarity of the NorM sequence to those of putative proteins in sequence databases, it seems that Na+/drug antiporters are present not only in V. parahaemolyticus but also in a wide range of other organisms.

* Corresponding author. Mailing address: Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, 700-8530, Japan. Phone and fax: 81-86-251-7957. E-mail: tsuchiya{at}pharm.okayama-u.ac.jp.

Journal of Bacteriology, December 2000, p. 6694-6697, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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