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Journal of Bacteriology, December 2000, p. 6724-6731, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Inducible Metabolism of Phenolic Acids in Pediococcus pentosaceus Is Encoded by an Autoregulated Operon Which Involves a New Class of Negative Transcriptional Regulator

Lise Barthelmebs, Bruno Lecomte, Charles Divies, and Jean-François Cavin*

Laboratoire de Microbiologie UMR-INRA, ENSBANA, Université de Bourgogne, F-21000 Dijon, France

Received 19 April 2000/Accepted 21 September 2000

Pediococcus pentosaceus displays a substrate-inducible phenolic acid decarboxylase (PAD) activity on p-coumaric acid. Based on DNA sequence homologies between the three PADs previously cloned, a DNA probe of the Lactobacillus plantarum pdc gene was used to screen a P. pentosaceus genomic library in order to clone the corresponding gene of this bacteria. One clone detected with this probe displayed a low PAD activity. Subcloning of this plasmid insertion allowed us to determine the part of the insert which contains a 534-bp open reading frame (ORF) coding for a 178-amino-acid protein presenting 81.5% of identity with L. plantarum PDC enzyme. This ORF was identified as the padA gene. A second ORF was located just downstream of the padA gene and displayed 37% identity with the product of the Bacillus subtilis yfiO gene. Subcloning, transcriptional analysis, and expression studies with Escherichia coli of these two genes under the padA gene promoter, demonstrated that the genes are organized in an autoregulated bicistronic operonic structure and that the gene located upstream of the padA gene encodes the transcriptional repressor of the padA gene. Transcription of this pad operon in P. pentosaceus is acid phenol dependent.

* Corresponding author. Mailing address: Laboratoire de Microbiologie UMR-INRA, ENSBANA, 1 Esplanade Erasme, F-21000 Dijon, France. Phone: (33) 03.80.39.66.72. Fax: (33) 03.80.39.66.40. E-mail: cavinjf{at}u-bourgogne.fr.

Journal of Bacteriology, December 2000, p. 6724-6731, Vol. 182, No. 23
0021-9193/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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